摘要
目的 建立毛细管区带电泳测定血浆中丙戊酸钠的方法。方法 血浆标本经HCl酸化，正己烷萃取，再以NaOH溶液反提取丙戊酸钠。采用75μm（id）×37cm石英毛细管柱分离。电泳缓冲液为15mmol／L pH5．7水杨酸钠溶液（含0．5mmol／L CTAB，15％乙醇），分离电压20kV，电泳时间6min，温度20℃，阴极压力进样5s，阳极检测，波长214nm。结果 血药浓度线性范围25-200μg／ml，r=0．999，最低检测浓度0．35μg/ml（S／N=3），萃取回收率87．4％，方法回收率100．1％，日内、日间变异系数（CV）分别小于4％、6％。结论 本方法可以简便、快速、灵敏地测定血浆中丙戊酸钠浓度。
Objective To establish a rapid determination of sodium valprate in plasma by capillary zone electrophoresis. Method Plasma was acidized by 1 mol/L HCl, VPA was extracted into organic phase （n-Hexane）, then re-extracted into aqueous phase （0. 05 mmol/L NaOH solution including 15% Ethanol）. Capillary clone was 75 μm（id） × 37 cm. Electrolyte consisted of 15 mmol/L sodium salicylate, 0. 5 mmoL/L CTAB and 15% Ethanol （pH 5.7）. Separating voltage was 20 kV, detection wave was 214 nm, temperature was 20℃ ,injection time was 5 s by press in negative. Result The linear ranger of concentration for standard drug was between 25 - 200 μg/ml （r = 0.999）, the limit of detection was 0.35 μg/ml, the average recovery of VPA was 87.4% ,the average inter-day and intar-day CV were less than 4% and 6%. Conclusion This method is simple, rapid, reliable and correct, for determination of VPA in plasma.
出处
《中华检验医学杂志》
CAS
CSCD
北大核心
2006年第2期164-166,共3页
Chinese Journal of Laboratory Medicine
关键词
丙戊酸钠
血药浓度
电泳
毛细管
Sodium valprate
Plasma concentration
Electrophoresis,capillary
