摘要
用气相色谱仪进行血清中丙戊酸钠浓度的测定。将含有丙戊酸钠的血清样品酸化，而后加入含有内标正乙酸的氯仿，采用２ｍ长不锈钢色谱柱，检测器ＦＩＤ，固定液为质量分数１５％ＤＥＧＳ，氯仿抽提血中丙戊酸钠。结果：丙戊酸钠浓度在０．０５６～１．８１０ｍｍｏｌ／Ｌ间线性关系良好，最低检测限为６．０２×１０－３ｍｍｏｌ／Ｌ，变异系数２．０％，回收率９７％。提示：该方法简单可靠，重复性好，可作为患者合理用药的一个定量依据。
A sensitive gas chromatographic method was used to determine sodium valprate concentration in the serum of patients with epilepsy during administration of the drug.Sodium valprate in serum was extracted with chloroform containing caproic acid and acidified with HCl.The extract was analysed with stainless steel column filled in 15% DEGS with FID.The standard curve was linearly correlated to serum drug concentration of the patients between 0.056 to 1.81 mmol/L.The detective limit was 6.02×10-3 mmol/L,CV was 2.0%,and recovery was more than 97.0%.The method is simple and reliable.The results of the guantative analysis can be suitable for the patients correct administration.
出处
《河南医科大学学报》
1997年第4期78-80,共3页
Journal of Henan Medical University
关键词
丙戊酸钠
气相色谱
血药浓度
gas chromatography
sodium valprate
drug concentration in serum
