摘要
[目的]优化灵芝中三萜化合物HPLC法的分离条件。[方法]用RP-HPLC法,考察不同提取剂、不同梯度洗脱、不同进样量对灵芝中三萜类化合物分离的影响,同时在最佳条件下分离测定4种市售保健品中三萜类组分。[结果]灵芝中三萜化合物提取剂为氯仿;优选出的灵芝三萜组分的HPLC分离条件为:流动相A为0.2%乙酸-水,流动相B为乙腈,梯度洗脱;检测波长为254nm;进样量为10μl;市售的4种灵芝保健品中只有2种保健品的HPLC分离图谱能与灵芝原药材对应。[结论]该试验得到分离灵芝中的三萜类组分的最佳分离条件,同时对4种市售保健品中三萜类组分进行了测定,此法可用于灵芝提取物的质量控制和其保健品的鉴定。
[Objective]The aim was to optimize the separation condition of triterpenoids from Ganoderma lucidum. [Method]The effects of different extraction solvents,different mobile phases and different injecting amount on the separation of triterpenoids were studied by RP-HPLC,and the triterpenoids of 4 kinds of health care products were determined under the optimum condition. [Result]The optimum condition were as follows：extraction solvents of chloroform,aqueous phase of 0.2% acetic acid-water,injecting amount of 10-30 ul,and the chromatogram of 2 kinds of the health care products were similar with that of Ganoderma lucidum medicinal material. [Conclusion]The optimum conditions were obtained,and the 4 kinds of health care products were determined by this method,which could used to control the extraction quality of Ganoderma lucidum and evaluate the health care products.
出处
《安徽农业科学》
CAS
北大核心
2010年第24期13029-13031,共3页
Journal of Anhui Agricultural Sciences
基金
2009年湖南省高校科学研究项目(09C1212)
