摘要
目的研究甲基硒酸对肾癌GRC-1细胞的生长抑制作用及与线粒体融合蛋白2的关系。方法用不同浓度(1.0μmol/L、2.5μmol/L、5.0μmol/L)的甲基硒酸对GRC-1细胞进行侵染,通过四甲基偶氮唑盐(MTT)比色法检测亚硒酸钠对人脑胶质瘤干细胞的增殖的影响,RT-PCR法检测Mfn2的表达,荧光检测仪检测GRC-1细胞内ROS含量。结果甲基硒酸对肾癌GRC-1细胞具有明显的抑制作用,且随甲基硒酸浓度的增加,GRC-1细胞生长抑制作用逐渐加强,中、高剂量染硒组差异具有统计学意义(P<0.05);Mfn2的表达在高剂量染硒组显著上调(P<0.05);GRC-1细胞内ROS含量在低、中剂量染硒组均显著性增加(P<0.05)。结论甲基硒酸能抑制肾癌GRC-1细胞的生长,Mfn2可能参与到其作用机制中。
Objective To investigate effects of methylseleninie acid on the GRC-1 growth inhibi- tion and the relationship of mitofusin-2. Methods Human renal cell carcinoma ceils （GRC-1） were trea- ted with different concentrations（1.0ttmol/L, 2. 5μmol/L, 5.0ttmol/L）of methylseleninic acid. Cell growth inhibition of GRC-1 was detected by assay of MTT. Expression of Mfn2 was tested by poly- merase chain reaction. Fluorescence probe was used to label GRC-1, intracellular reactive oxygen spe- cies were observed with a fluorescence detector device. Results Methylseleninic acid can significantly suppressed the growth of GRC-1. The cell growth rate was decreasing with the dose of methylseleninic acid increasing. There were difference in statistical significance in the middle and the high groups（P〈 0. 05）. Expression of Mfn2 was increased significantly in 5.0μmol/L groups（P〈0.05）. Intracellular reactive oxygen species was increased significantly in 1.0btmol/L and 2.5μmol/L groups （P〈0.05）. Conclusion Methylseleninic acid suppressed the growth of GRC-1, and Mfn2 may be involved in this mechanism.
出处
《贵州医药》
CAS
2012年第8期679-682,共4页
Guizhou Medical Journal
基金
贵州省卫生厅科学技术基金资助项目[GZWKJ2011-1-084]
关键词
甲基硒酸
肾癌
线粒体融合蛋白2
Methylseleninic acid Renal cell carcinoma Mitofusin-2
