摘要
目的探讨槟榔碱对3T3-L1前脂肪细胞增殖、分化的影响及其机制。方法采用四甲基偶氮唑盐(MTT)法和油红O染色检测不同浓度(10、30、50、100μmol/L)的槟榔碱对3T3-L1前脂肪细胞增殖和分化的影响,荧光定量PCR检测过氧化物酶体增殖物激活受体γ(PPARγ)和CAA T/增强子结合蛋白α(C/EBPα)的表达。结果 10～100μmol/L槟榔碱作用3T3-L1前脂肪细胞12 h和24 h能促进3T3-L1前脂肪细胞增殖,且呈一定的量效关系。到分化的第9天,与对照组相比,50μmol/L槟榔碱组胞浆中的脂滴明显减少,同时PPARγ和C/EBPαmRNA表达水平显著降低。结论槟榔碱能促进3T3-L1前脂肪细胞的增殖、抑制其分化,其机制可能与PPARγ和C/EBPα的表达降低有关。
objectives To investigate the effects of arecoline on the proliferation and differentiation of 3T3-L1 pread- ipocytes and to elucidate its possible mechanism. Methods 3T3-L1 preadipocytes were cultured and treated with differ- ent concentrations of arecoline （ 10,30,50,100 μmol/L） , the proliferation and differentiation of 3T3-L1 preadipoeytes were evaluated by MTTmethods and oil red O staining ,respectively. The expressions of peroxisome proliferation activated receptorα （PPAR α） and CAAT/ enhancer binding protein （C/ EBP α） mRNAs were detected by quantitative real-time RT-PCR （qRT-PCR）. Results Treatment of arecoline at different concentrations （ 10 - 100 μ mol/L） for 12 and 24 h signifi- cantly promoted 3T3-L1 preadipocyte proliferation in a dose-depend manner. On the day 9 of differentiation, compared with the control group, lipid droplets in the cytoplasma and the PPARγ and C/EBP α mRNA expression were significantly de- creased in the 50 μmol/L arecoline group. Conclusion Arecoline promotes the proliferation and inhibits the differentia- tion of 3T3-L1 preadipocytes, which may be associated with decreased expressions of PPAR γ and C/EBP α mRNA.
出处
《中南医学科学杂志》
CAS
2013年第1期7-9,27,共4页
Medical Science Journal of Central South China
基金
国家自然科学基金(81000328)
南华大学博士科研启动基金(2010XQD39)资助
