摘要
目的:探讨槟榔碱对2型糖尿病大鼠β细胞分泌功能及胰腺十二指肠同源盒因子-1(pancreas-duodenum homeobox-1,PDX-1)mRNA表达的影响。方法:采用高果糖饲料饲养Wistar大鼠12周制备2型糖尿病大鼠模型,40只实验动物随机分为5组:对照组、模型组、模型+1 mg/kg槟榔碱组、模型+5 mg/kg槟榔碱组、模型+10 mg/kg槟榔碱组。药物注射4周后股动脉取血检测空腹血糖、三酰甘油、总胆固醇、高密度脂蛋白、低密度脂蛋白、血清胰岛素,处死大鼠取胰腺组织,石蜡切片HE染色观察组织形态学变化,RT-PCR检测β细胞内PDX-1及胰岛素mRNA的表达水平。结果:(1)高糖作用引起Wistar大鼠胰腺β细胞PDX-1及胰岛素mRNA的表达水平显著下降(P<0.01);(2)1 mg/kg和5 mg/kg槟榔碱处理能显著上调高糖喂养Wistar大鼠胰腺β细胞PDX-1与胰岛素mRNA表达水平(P<0.05,P<0.01)。结论:槟榔碱可以通过上调PDX-1和胰岛素基因表达,改善高糖环境下的β细胞胰岛素合成和分泌功能的损伤。
Objective To investigate the effect of arecoline on insulin secretion and the mRNA expression of pancreas-duodenum homeobox-1（PDX-1） in β cells in rats with Type 2 diabetes meilitus.Methods Wistar rats were fed with high-fructose diet for 12 weeks to induce Type 2 diabetes meilitus.Forty Wistar rats were randomly divided into 5 groups： a control group;a diabetic group;and a low（1 mg/kg）,medium（5 mg/kg）,or high（10 mg/kg） dose of arecoline groups.Fasting blood glucose（FBG）,triglyeride（TG）,total cholesterol（TC）,high density lipoprotein（HDL）,low density lipoprotein（LDL）,and fasting serum insulin（FSI） were measured after treatment with arecoline for 4 weeks.Pancreatic tissue was fixed in 10% formaldehyde,sliced and HE dyed.RT-PCR was used to detect the mRNA expression of PDX-l and insulin.Results（1） Insulin secretion and mRNA levels of PDX-1 were significantly decreased in rats with Type 2 diabetes meilitus compared with the control group.（2） Compared with the diabetic group,insulin secretion and mRNA levels of PDX-1 were significantly increased in the medium and high dose of arecoline groups.Conclusion Arecoline can prevent the dysfunction of β cells of pancreas induced by high fructose via up-regulation of PDX-1.
出处
《国际病理科学与临床杂志》
CAS
2010年第1期14-19,共6页
Journal of International Pathology and Clinical Medicine
基金
湖南省科学技术厅项目(2006TP3016
2007TP4018)~~
