摘要
目的 探讨美解眠致大鼠化学点燃性癫痫有效剂量 ,建立化学点燃模型。方法 雄性Wistar大鼠 ,分 7个实验组和对照组 ,依照寇氏法安排美解眠剂量 ,腹腔注射后观察行为发作并判定级别 ,同时记录脑电图。记录首次抽搐发作平均时间 (SB) ,第一次达到V级发作平均时间 (RFSvS) ,达点燃标准平均时间(RKC)。结果 美解眠致癫痫ED97为 18 74mg/kg ,SB为 2 13± 10 0秒 ,RKC 2 88± 10 9秒 ,点燃率为 86 7%。结论 本实验推荐美解眠 18 7mg/kg为大鼠化学点燃腹腔注射致痫剂量。
Objective To establish a kindling model of epilepsy induced by intraperitoneal injection of bemegride in rats and study its effective dosage.Methods Adult male Wistar rats were divided into 7 groups,and the doses of bemegride for estimating kindling epilepsy were scheduled according Krber's method.The intensity of behavioral seizures was evaluated by the use of the six_point scoring system,and EEG was recorded.Mean time of seizure beginning(SB),reaching the first stage V seizure (RFSvS) and reaching the kindling criterion (RKC) were observed.Results Effective dose of induced epilepsy (97% of animals) was drawn 18.74 mg/kg.The mean of SB and RKC were 213±100 seconds and 288±109 seconds respectively.The kindling rate was 86.7%. Conclusion In the experimental condition,it is optimal to select 18.7 mg/kg as the dosage of bemegride for establishing kindling model.
出处
《兰州大学学报（医学版）》
CAS
2001年第3期14-17,共4页
Journal of Lanzhou University（Medical Sciences）
关键词
癫痫
化学点燃模型
美解眠
动物模型
有效剂量
Epilepsy Chemical kindling model Bemegride Animal model Effective dose
