摘要
目的:研究清热解毒中药对心肌细胞缺氧复氧损伤影响的药效学,探讨其治疗冠心病心肌缺血的作用机制。方法:复制心肌细胞缺氧复氧损伤模型,以LDH、MDA、SOD、TNF-α、IL-6、NO、NOS为观测指标,以地尔硫卓为平行对照,观察清热解毒中药干预冠心病心肌缺血的作用机制。结果:清热解毒中药能降低缺氧复氧损伤心肌细胞培养液中LDH活力和MDA含量,升高SOD活力(P<0.01或P<0.05);能降低缺氧复氧损伤心肌细胞培养液中TNF-α、IL-6、NO和NOS(P<0.01或P<0.05)。结论:清热解毒中药抗冠心病心肌缺血作用机制是通过抑制炎性因子TNF-α、IL-6的表达,并提高过氧化物歧化酶SOD活力,减少氧自由基的产生和MDA的形成,抗脂质过氧化损伤,保护缺血心肌。
Objective： To study the pharmacodynamics of antipyretic - detoxicate traditianal TCM of interference on hypoxia - reoxygenation injury in myocardial cell, and explore the mechanism of drugs in treatment of myocardial ischemia. Method： The LDH MDA, SOD, TNF -α, IL - 6, NO, NOS were detected in the rat models of acute myocardial ischemic injury, and the dihiazem was parallel contrast. Result： The level of SOD were significantly increased, and the levels of MDA and LDH were significantly decreased in the culture solution by the antipyretic - detoxicate TCM （ P 〈 0. 01 or P 〈 0. 05 ） ; The levels of TNF -α, IL - 6 , NO and NOS in the culture solution were significantly decreased by the antipyretic -detoxicate TCM （P 〈0. 01 or P 〈0. 05 ）. Conclusion： The mech- anism of action of the antipyretic - detoxicate traditional Chinese medicine on myocardial ischemia is that the delivery of TNF - α and IL - 6 in the blood serum was restrained and the action of vascular endothelium was improved to restrain the inflammatory reaction on myocardial ischemia.
出处
《中国民族民间医药》
2013年第2期34-36,共3页
Chinese Journal of Ethnomedicine and Ethnopharmacy
关键词
清热解毒中药
心肌细胞
冠心病
缺氧复氧
The antipyretic -detoxicate TCM
Myocardial cell
Coronary heart disease
Hypoxia- reoxygenation
