摘要
目的:研究巴戟天对纯化培养心肌细胞缺血再灌注损伤的防护作用及机制。方法:采用纯化培养的心肌细胞建立缺氧复氧损伤模型,实验分5组:正常细胞培养组,缺氧复氧损伤模型组,巴戟天大、中、小剂量组。分别用黄嘌呤氧化酶法测定SOD活力,硫代巴比妥酸显色法测定MDA含量,测定LDH活性以及NO含量变化。结果:巴戟天正丁醇可溶部分可明显提高SOD、LDH活性,降低MDA含量、增加NO含量。结论:巴戟天具有明显的抗缺氧复氧损伤、保护心肌作用。
Objective: To make a research into the protecting effect of Ba Ji Tian on the reputing injury of purified cultural hypoxia of cardiac muscle cell. Method: The models of purified cultural hypoxia of cardiac muscle cell were made and divided into five groups: normal cultural group, the group of hypoxia of cardiac muscle cell, three Ba Ji Tian groups of high dosage, medium dosage and low dosage. Their SOD, the content of MDA, LDH and NO were separately tested. Result: Ba Ji Tian could distinctiely raise SOD and LDH, lower MDA and increase NO. Conclusion: Ba Ji Tian is markedly effective in protesting cardiac muscle.
出处
《河南中医学院学报》
CAS
2005年第3期20-21,共2页
Journal of Henan University of Chinese Medicine
