摘要
目的:研究玉米花粉黄酮提取物对模拟空间辐照小鼠的血液及肝脾等脏器的防护作用。方法:将小鼠随机分成5组:正常对照组、辐射对照组、芦丁阳性组、玉米花粉黄酮40%乙醇提取物给药组(FMP-1组)、玉米花粉黄酮95%乙醇提取物给药组(FMP-2组)。每组10只,均灌胃给药,FMP-1,FMP-2及芦丁组小鼠灌胃剂量依次为78、54、78mg/kg/d,空白对照组及辐照对照组以10mL/kg/d剂量灌服蒸馏水。灌胃21d后,通过医用电子直线加速器进行4.0Gy剂量模拟空间辐照,辐照后24h后处死。对肝脾指数及血液、肝脏、脾脏中SOD、GSH-Px活力及MDA含量测定。结果:与辐照对照组相比,玉米花粉黄酮40%、95%乙醇提取物均能显著提高辐照小鼠血液、脾脏的SOD活力、GSH-Px活力(p<0.05),且能显著降低组织中MDA含量(p<0.05),且两组各指标均接近空白对照组指标。结论:玉米花粉黄酮40%及95%乙醇提取物均能对空间辐射引起的血液及免疫系统等组织器官危害起到很好的防护作用。
Objective：To study protective effect of corn pollen flavonoids on blood,liver and spleen of imitate space radiation injured in mice.Methods：Mice were randomly divided into 5 groups,include normal control group,negative control group,positive control group,FMP-1 group and FMP-2 group,10 mice in each group.The mice of positive control group,FMP-1 group and FMP-2 group were administered intragastrically with Rutin（78mg/kg/d）,FMP-1（78mg/kg/d）and FMP-2（54mg/kg/d）respectively,normal control group and negative control group were administered intragastrically with deionized water（15mL/kg/d）.The animals of all groups except normal control group were treated by imitate space irradiation after gavage 21d.The radiation dose was 4Gy.24h after irradiation animals were sacrificed.The internal organs,include blood,liver and spleen,were dissected out from each animal and used for biochemical investigations.Results：Compared with the negative control,the SOD and GSH-Px activity of the FMP-1 and FMP-2 groups increased significantly in internal organs（p0.05）,and MDA levels decreased significantly（p0.05）.Conclusion：Corn pollen extracts,expecially the crude flavonoids extract,had some ideal protective effect against imitate space radiation injury in mice and might be a ideal natural antioxidant potentially.
出处
《食品工业科技》
CAS
CSCD
北大核心
2013年第1期350-353,共4页
Science and Technology of Food Industry
