摘要
目的:优选野蔷薇根中熊果酸的超声提取工艺。方法:使用超声波提取法,采用L(934)正交设计试验,用高效液相色谱法测定熊果酸的含量,确定最佳提取工艺条件。结果表明:野蔷薇根中熊果酸的提取最佳工艺是超声功率为600W,超声频率为工作5s间歇5s,料液比1∶25,提取时间为30min,各因素的影响依次为超声波频率>超声波功率>料液比和提取时间。结论:超声辅助提取野蔷薇根中熊果酸是一种简单、快捷、高效的提取方法。
Objective： to optimize the ultrasonic extraction ofursolic acid in Rosa multitlora Thumb. Method： utilizing the ultrasonic extraction method and using the orthogonal test ofL9（3^4）, with high-performance liquid chromatography （HPLC） to determine ursolic acid content and the optimum ex- traction conditions. The results showed that the order of each factor affecting the extraction was ultrasonic frequency, ultrasonic power, the ratio of liquid to material and extracting time. The optimum conditions were as follows： ultrasonic power 600W, ultrasonic frequency of resting for 5s and working for 5s, the ratio of liquid to material 1：25 and extracting time 30min. Conclusion： the ultrasonic-assisted extracting of ursolic acid from the root of wild roses is a simple, quick and efficient way.
出处
《中国酿造》
CAS
2012年第12期41-43,共3页
China Brewing
基金
国家自然科学基金(21165018)资助
关键词
野蔷薇
熊果酸
超声提取
HPLC法
Rosa multiflora
ursolic acid
ultrasonic extraction
HPLC method
