摘要
目的:研究新疆地产植物野蔷薇、黑加仑、沙枣、巴旦杏提取物对人肝癌细胞的抑制作用。方法:将野蔷薇、黑加仑、沙枣、巴旦杏分别分为高、中、低剂量组(10-1、10-2、10-3g/ml),另设阳性(顺铂)对照组、阴性对照组(含同一细胞密度的培养液)、溶剂对照组(7.5%甘油),在人肝癌细胞中加入不同剂量浓度的受试物,分别于24、48、72 h在倒置显微镜下观察肿瘤细胞的生长情况,通过MTT比色法测定OD值。结果:与阴性对照组比较,野蔷薇高剂量和黑加仑72 h、沙枣和巴旦杏48 h对肿瘤细胞QCY-7404的抑制作用最强,野蔷薇和黑加仑的抑瘤作用随时间的延长而加强,而沙枣和巴旦杏的抑瘤作用与时间没有依赖关系。结论:野蔷薇、黑加仑、沙枣和巴旦杏提取物高剂量对肿瘤细胞QCY-7704有明显的抑制作用。
A Objective： To study the inhibitory effect of human liver cancer cell by extractions from Xinjiang area plants multiflora rose, black current, narrow-leaved oleaster, almond tree. Methods： There were high, middle and low dosage groupe （10 ^-1 , 10^-2, 10^-3g/ml）, positive （cis platinum） control group, negative control group （including identical cell density nutrient fluid）, solvent control group （7.5% glycerine） ,adds the different density of the dosage to the human liver cancer cell and keep in 24 h, 48 h and 72 h, separately. Then the situations of the tumor cell growth under the inverted microscope werd observed and the OD values through the MTT colorimeter were recorded. Results： The high dose （10 ^-1 g/L） of the multiflora rose and black current which keep in 72 h, the black current and the amygdalus communis which keep in 48 h were the best to suppress the tumor cell of QCY-7404, compare the ends with the negative control group, at the same time its OD value respectively were： 0. 196±0. 030, 0. 156±0. 021, 0. 243± 0. 024, 0. 377±0. 022 respectively （P〈0.01）. Conclusion： Multiflora rose, black current, narrow-leaved oleaster, almond tree extraction have obvious effects to inhibit the growth of the tumor cell QCY-7704.
出处
《新疆医科大学学报》
CAS
2008年第7期828-830,共3页
Journal of Xinjiang Medical University
关键词
野蔷薇
黑加仑
沙枣
巴旦杏
抗肿瘤
multiflora rose
black current
narrow-leaved oleaster
almond tree
anti-tumor
