摘要
目的 :探讨化疗前后卵巢癌细胞及外周血淋巴细胞凋亡率及凋亡因子Fas和Apo2 7的含量及其临床意义。方法 :从 75例化疗前后卵巢癌患者的腹水中提取富含癌细胞的悬液 ,用AnnexinV -FITC/PI双染法分辨凋亡细胞及坏死细胞 ,并以两种凋亡因子单克隆抗体Fas和Apo2 7标记上述细胞 ,均用流式细胞仪检测。结果 :化疗后卵巢癌细胞凋亡率显著高于化疗前的临床疗效好 ,反之疗效差 ;被Fas及Apo2 7标记的癌细胞率显著增加者临床疗效好 ,反之疗效差 ;化疗后外周血淋巴细胞凋亡率及凋亡因子有不同程度的增加。结论 :通过对化疗前后卵巢癌细胞凋亡程度的检测 ,有助于判断化疗效果 ,有重要的临床意义。
Objective: To Study the apoptotic rates (AR) and the contents of CD 95 (Fas factor) and Apo 2 7 by FCM in ovarian cancer cells(OCC) and its peripheral blood lymphocytes (PBL) as well as the clinical significance Methords: Ovarian carcinoma cell(OCC) suspensions were collected from ascites samples in 75 patients with advanced ovarian carcinomas before and after chemotherapy respectively The samples of OCC were painted by Annexin V FITC/Propidium iodide(PI) and assayed its AR, which could distinguished early apoptotic cells from necrotic cells The ATC suspensions were also labelled by monoclonal antibodies of CD 95 and Apo 2 7 Results: The curative effect in which patients whose AR, as well as the contents CD 95 and Apo 2 7 of OCC become statistically more increased after chemotherapy than before was satisfied;the AR as well as the contents of CD 95 and Apo 2 7 of PBL also become increased in different degree Conclusions: The assay of OCC apoptotic degree before and after chemotherapy is help to indicate the curative effect for ovarian carcinoma,and has important clinical significance
出处
《癌症》
SCIE
CAS
CSCD
北大核心
2000年第4期383-386,共4页
Chinese Journal of Cancer
关键词
卵巢肿瘤
细胞凋亡
药物疗法
流式细胞术
Ovarian tumor
Apoptosis
Chemotherapy
Monoclonal antibody
CD 95
Apo 2 7
