摘要
目的 探讨化疗前后卵巢癌细胞及外周血淋巴细胞凋亡率及凋亡因子Ｆａｓ和Ａｐｏ ２．７含量及其临床意义。方法 从６８ 例化疗前后的卵巢癌患者腹水中提取富含癌细胞的悬液，用Ａｎｎｅｘｉｎ Ｖ／ＰＩ双染法分辨凋亡细胞，并用２种凋亡因子单克隆抗体标记上述细胞，均用流式细胞仪检测。结果 化疗后卵巢癌细胞凋亡率显著高于化疗前（Ｐ＜ ０．０１），被Ｆａｓ及Ａｐｏ ２．７标记癌细胞率也显著性增加（Ｐ＜ ０．０５）；化疗使外周血淋巴细胞凋亡率及凋亡因子有不同程度的增加；结论 通过对化疗前后卵巢癌细胞凋亡的检测有助于判断化疗效果，有重要的临床意义。
? Objective To investigate the apoptotic rate (AR) and the contents of CD 95 (Fas factor )and Apo 2.7 in ovarian cancer cell(OCC) and its peripheral blood lymphocytes (PBL) before and after chemotherapy and its clinical significance.Method The autologue tumor cell suspensions from ascites samples of 68 ovarian cancer patients has collected before and after chemotherapy to be stained with Annexin V FITC/Propidium iodide (PI) or to be labelled with the monoclonal antibodies of CD 95 and Apo 2.7,all to be assayed by FCM.Results The apoptotic rate of ovarian cancer after chemotherapy was significantly higher than that before chemotherapy (P<0.01),and the cancer cells labeled by Fas and Apo 2.7 also increased significantly(P<0.05).With chemotherapy,PBL′s apoptotic rates and apoptotic factor increased in differnt degree.Conclusion The apoptotic degree of ovarian cancer cell before and after chemotherapy is used for indicating the effect of chemotherapy,and also has important clinical significance. 〔
出处
《中国肿瘤临床与康复》
1999年第4期21-23,共3页
Chinese Journal of Clinical Oncology and Rehabilitation
关键词
卵巢肿瘤
凋亡
化学疗法
Ovarian tumor
Apoptosis
Chemotherapy
