摘要
目的 探讨不同剂量X射线照射对原代人脐静脉内皮细胞(HUVEC)和PC-3细胞周期和凋亡的影响,为临床放疗提供理论依据。方法 采用形态学观察和Annexin V-FITC联合PI双染法和ApO2.7单抗法流式细胞仪(FCM)定量检测原代HUVEC和PC-3细胞在0-10 Gy的6MV-X射线照射后细胞周期和凋亡的变化。结果 照射后24 h和48 h两种细胞均出现明显G0/G1期减少和G2/M期增加,但2 Gy照射时仅对PC-3细胞周期有影响;两种细胞照射后均未出现辐射相关的凋亡。结论 电离辐射可诱导两种细胞出现G2/M期阻滞但不引起凋亡发生,两种细胞均有一定的辐射抗拒性。
Objective To preliminarily investigate the effect of various dose of radiation on Cell Cycle and apoptosis of primary human umbilical vein endothelial cells(HUVECs) and PC - 3 cells.Methods Morphology and flow cytometry(FCM)were used to detect changes of cell cycle and apoptosis in HUVECs and PC - 3 cells radiated with 6MV X - ray.Results At hour 24 or 48 after radiation,changes of cell cycle in HUVECs and PC - 3 cells were detected but no apoptosis was induced. Conclusion Radiation can induce retardation of cell cycle but no apoptosis in primay HUVECs and PC - 3 cells, it suggests that the two kinds of cells have part resistance against radiation.
出处
《河南肿瘤学杂志》
2003年第1期16-18,共3页
Henan Journal of Oncology
