摘要
目的探讨用实体瘤组织制备膜完整的单细胞悬液、并与ＣＤ４４荧光单克隆抗体反应的理想方法。方法应用流式细胞免疫学方法，对１５种共７８例不同组织来源的良恶性肿瘤手术切除组织的细胞进行了粘附分子ＣＤ４４定量研究。结果恶性肿瘤组（６２例）组织内ＣＤ４４含量明显高于良性肿瘤组（１６例，Ｐ＜０．０５）及外周血淋巴细胞ＣＤ４４含量（Ｐ＜０．０５）；良性肿瘤组织ＣＤ４４含量与外周血淋巴细胞ＣＤ４４含量相近（Ｐ＞０．０５）。
Objective To study the method making cellular suspension with intact membrane from solid tumor tissues for the immunological reaction with fluorence monoclonal antibody CD 44 Methods The authors studied 78 solid tumor samples of fifteen kinds of varied benign and malignant tumors and their adhesive molecule CD 44 contents by flow cytometric immunology (FCMI) technique Results The CD 44 contents in malignant tumors tissues were statistically more than those in benign ones ( P <0 05) and peripheral lymphocytes ( P <0 05). The CD 44 contents in benign tumor tissues,however,were statistically similar to those in peripheral lymphocytes( P >0 05) Conclusion This study suggests that the CD 44 contents in solid tumor tissues measured by FCMI may play an important role in differentiating benign from malignant nature of tumors
