摘要
目的 探讨二硫化碳 (CS2 )对邻苯三酚 鲁米诺 (PA L)自氧化化学发光体系以及对超氧阴离子产生的影响。方法 应用PA L化学发光体系观察CS2 的发光动力学曲线及超氧化物歧化酶(SOD)对发光强度的抑制作用。结果 不同浓度CS2 (10、40、80mg/ml)与乙醇溶剂对照组比较 ,均能使PA L体系化学发光强度增强 ,峰时延迟 ,呈明显的剂量 效应关系 ,其中CS2 浓度为 80mg/ml时化学发光峰值高于无溶剂本底值 ,CS2 化学发光均可被SOD所抑制。结论 CS2 可在PA L体系中产生超氧阴离子 。
Objective To study the effect of carbon disulfide (CS 2) on pyrogallol luminol auto oxidation chemiluminescence (PA L) system and production of superoxide anion Methods Luminescence dynamic curve of CS 2 and inhibition effect of superoxide dismutase (SOD) on intensity of chemiluminescence induced by CS 2 were studied with PA L chemiluminescence system. Results Different concentrations of CS 2 (10、40、80 mg/ml) could enhance the emission intensity of chemiluminescence of PA L system and delayed its peak time, with a significant dose effect relationship, as compared with that of solvent ethanol Peak of chemiluminescence induced by 80 mg/ml of CS 2 was higher than its background value without solvent ethanol Chemiluminescence induced by CS 2 could be inhibited by SOD Conclusion CS 2 can generate superoxide anion in PA L system and delay the peak time of chemiluminescence emission dynamic curve
出处
《中华预防医学杂志》
CAS
CSCD
北大核心
2000年第3期162-164,共3页
Chinese Journal of Preventive Medicine
关键词
二硫化碳
化学发光
邻苯三酚
超氧阴离子
Carbon disulfide
Chemiluminescence
Pyrogallol
Anions
Superoxide dismutase
