摘要
目的研究甲醛和甲苯联合染毒对小鼠脑细胞的损伤作用,探讨其联合毒性效应的类型。方法选择健康清洁级雄性昆明小鼠36只,按3×3析因设计,将小鼠随机分为9组,每组4只,即对照组(0 mg/m3甲醛+0 mg/m3甲苯),甲醛组(1、5 mg/m3),甲苯组(400、2 000 mg/m3),联合组(1 mg/m3甲醛+400 mg/m3甲苯、1mg/m3甲醛+2 000 mg/m3甲苯、5 mg/m3甲醛+400 mg/m3甲苯、5 mg/m3甲醛+2 000 mg/m3甲苯)。采用静式吸入染毒,将小鼠暴露于不同浓度的甲醛、甲苯及两者的混合气体中,每天2 h,连续14 d。染毒结束后通过彗星试验测定小鼠脑细胞DNA损伤情况。结果甲醛和甲苯单独及联合染毒可致脑细胞彗星尾部DNA含量增高,尾矩增大,差异有统计学意义(P<0.05),其中低甲醛和低甲苯联合染毒组尾部DNA含量(37.96%)和尾矩(21.38)最大;二者联合染毒致小鼠脑DNA损伤存在交互作用(P<0.05),且表现为协同。结论甲醛和甲苯可致小鼠脑细胞DNA损伤,二者联合染毒具有一定的协同作用。
Objective To study the combined effect of exposure to formaldehyde and toluene on DNA damage in brain of mice. Methods Thirty-six healthy clean-class Kunming mice were randomly divided into nine groups, the control group（0 mg/m3 formaldehyde＋0 mg/m3 toluene）, formaldehyde group （1, 5 rag/m3）, toluene group （400, 2 000 mg/m3）, combined group （1 mg/m3 formaldehyde＋400 mg/m3 toluene, 1 mg/m3 formaldehyde＋2 000 mg/m3 toluene, 5 mg/m3 formaldehyde＋400 mg/m3 toluene, 5 mg/m3 formaldehyde＋2 000 mg/m3 toluene）. 3x3 factorial analysis was used in the experiment. The treatments were conducted by static inhaling formaldehyde or（and） toluene, two hours per day for 14 days consecutively. The DNA damage in brain cells of the mice were detected with comet assay. Results When the mice were jointly exposed to formaldehyde and toluene, the percentage of tail DNA and tail moment in brain cells in exposure groups were higher than the control group （P〈0.05）. There was an interaction between formaldehyde and toluene on percentage of tail DNA and tail moment（P〈0.05）, and they have synergistic effect. Conclusion Formaldehyde and toluene may induce DNA damage in brain ceils of mice. The joint toxicity of formaldehyde and toluene is considered synergistic.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2012年第12期1085-1087,F0003,共4页
Journal of Environment and Health
关键词
甲醛
甲苯
联合染毒
彗星试验
Formaldehyde
Toluene
Joint exposure
Comet assay
