摘要
目的 研究乳兔雪旺细胞 (Schwann cell,SC)对成兔视神经挫伤修复的作用。 方法 建立成兔视神经挫伤模型 ,伤后 2 4h分别向伤眼玻璃体腔内注入 SC悬液 (A组 )、生理盐水 (B组 )各 0 .1ml。伤后不同时间点进行视网膜节细胞 (retinal ganglion cell,RGC)、轴突染色记数及闪光视觉诱发电位 (flash visualevoked potentials,FVEP)检测。 结果 伤后 4周 A、B组 RGC平均记数分别为 (19.89± 3.79) /mm和(12 .6 7± 4.12 ) /mm,轴突密度分别为 (945 6 9± 793) /mm2 和 (36 0 85± 2 85 ) /mm2 ,A组明显高于 B组(P<0 .0 1)。伤后 3d A组伤眼与健眼 FVEP幅值比由 48%上升至 88% ,8周时仍为 78% ,各时间点均明显高于 B组 (P<0 .0 1)。 结论 乳兔 SC能够提高成兔视神经挫伤后 RGC存活率 ,减轻轴突变性 ,显著促进视神经功能恢复 ,对视神经挫伤修复具有明显的促进作用。
Objective [WT5”BZ]To study the effects of neonatol rabbit Schwann cells (SC) on repair of optic contusion in adult rabbits. [WT5”HZ]Methods 24 h after the adult rabbit optic nerves was contused,0.1 ml of SC suspension (group A) and saline water (group B) were injected into the vitreous of injured eyes respectively.All the animals were studied by retinal ganglion cell (RGC) and axon counting,flash visual evoked potential (FVEP) tests at various intervals after injury. [WT5”HZ]Results At the 4th week after injury,the number of RGC was (19.89±3.79)/mm in group A and (12.67±4.12)/mm in group B,and the density of axons was (94 569±793)/mm 2 in group A and (36 085±285)/mm 2 in group B.There was dramatical difference between group A and B (P<0.01).The amplitude of FVEP wave of group A increased from 48% to 88% on the 3rd day after injury,and still dept 78% at the 8th week and group A was significantly higher than group B at various intervals (P<0.01). Conclusion SC are effective in promoting the repair of optic nerve contusion by increasing the survival rate of RGC,rescuing axons from degeneration,and dramatically promoting the function of the optic nerve. [WT5”HZ]
出处
《中华眼底病杂志》
CAS
CSCD
北大核心
2000年第2期91-93,共3页
Chinese Journal of Ocular Fundus Diseases
基金
全军九五医药卫生科研基金
关键词
病理学
雪旺氏细胞
疾病模型
视神经挫伤
WT5”BZ]Optic nerve/pathology
Eye injnries
Schwann cells
Neurons
Disease models,animal
