摘要
目的:观察体外培养的兔视网膜细胞在受到机械损伤后的形态变化。方法:建立乳兔视网膜细胞及与Schwann Cell(SC)共培养模型,采用机械刮损方法对视网膜细胞造成损伤,相差显微镜下连续24小时观察细胞对损伤的反应。结果:刮损后30分钟胶质细胞逐渐移向损伤区,分裂增殖并填补裸区。神经元突起在损伤后6小时开始再生,其生长方向受到周围环境影响,具有“可塑性”。与SC共培养时,损伤后3小时即可发现再生突起。结论:视网膜神经元突起具有一定再生潜力,胶质增生不是再生的绝对障碍,SC对突起再生具有促进作用。眼科学报2000;16:127-130。
Purpose: To investigate the morphological changes of cultured rabbit retinal cells after injury.
Methods: Mechanical scratch was done in nenatol rabbit retinal cell culture and in Schwann Cell(SC)/retinal cell colculture. The morphological changes of different sorts of cells after the injury were observed under the phase contrast microscope for 24h. Results: About 30min after injury, many glial cells moved to the blank region and proliferated to form gliosis. Axons of retinal neurons began to regenerate 6h post injury. The direction of regrowth was influenced by the environment. In the coculture, regeneration of axons was discerned only 3h after injury.
Conclusion: Axons of retinal neurons had strong capability of regeneration; Gliosis was not the absolute obstacle of regeneration; SC played a role in promoting the axon regeneration. Eye Science 2000; 16: 127 - 130.
出处
《眼科学报》
2000年第2期127-130,共4页
Eye Science
基金
国家自然科学基金资助
基金号:39900076
