摘要
目的研究颈，神经不同平面切断及不同时期对神经元逆行性退变的影响，为临床选择合适的时间和方法保护受损神经元提供实验依据。方法36只SD雌性大鼠随机分为三组：对照组、神经根部切断组和神经支部切断组。利用True Blue逆行示踪技术对各组大鼠背根神经节感觉神经元和脊髓前角运动神经元精确计数，并利用神经元特异性烯醇化酶（neuron specific enolase，NSE）免疫荧光双标技术检验标记神经元的活性。结果术后1周，神经根部切断组和神经支部切断组较对照组无明显的感觉和运动神经元数量变化。术后16周，神经根部切断组和神经支部切断组较对照组感觉和运动神经元减少，其中神经根部切断组较神经支部切断组减少更明显（P〈0．05）。且各组True Blue标记神经元全部被NSE免疫荧光双标。结论颈，神经根部切断和神经支部切断均发生感觉和运动神经元逆行性退变，且不同平面切断对神经元退变的影响有显著性差异。提示臂丛神经根性损伤后早期修复受损神经有利于防止神经元退变。
Objective To investigate the retrograde degenerating effects on moto- and sensory neurons of C7 nerve transection at different levels and at different time, and to provide the basis for the proper timing and methods to protect neurons from injury clinically. Methods Thirty-six adult female SD rats were randomly divided into the normal control group, nerve root level transection group and nerve branch level transection group, with 12 rats each. Retrograde tracing using True Blue was conducted to count the numbers of sensory neurons in the dorsal root ganglion of C7 roots and motoneurons in the anterior hem of the C7 spinal cord. Neuron activity was detected applying neuron specific enolase （NSE） immunohistochemistry. Results The numbers of dorsal root ganglion sensory neurons and spinal cord motoneurons were not significantly different among the three groups at 1 week. The numbers of sensory neurons and motoneurons decreased in the nerve root level transection group and nerve branch level transection group at 16 weeks when compare to those of the control, the numbers of sensory neurons and motoneurons in the nerve root level transection group being even lower（ P 〈 0.05）. Sensory neuron and motoneuron traced by True Blue in the two transection groups could all he double labelled by NSE immunofluroscene technique. Condusion Retrograde degeneration of anterior hem motoneurons and dorsal root ganglion sensory neurons was detected after C7 nerve transection at root level and at nerve branch level. The more proximal the transection level, the more prominent the retrograde degeneration. Reparation of impaired brachial plexus root earlier can prevent motoneuron loss.
出处
《中华手外科杂志》
CSCD
北大核心
2012年第6期366-369,共4页
Chinese Journal of Hand Surgery
基金
上海市周围神经显微外科重点实验室、卫生部手功能重建重点实验室开放课题
关键词
臂丛
神经元
荧光免疫测定
逆行变性
Brachial plexus
Neurons
Fluroimmunoassay
Retrograde degeneration
