摘要
目的探讨原浆型星形胶质细胞(protoplasmic astrocyte,PAS)和纤维型星形胶质细胞(fibrous astrocyte,FAS)对神经干细胞(neural stem cell,NSC)定向分化的调控作用。方法将4’,6-二脒基-2-苯基吲哚(4’,6-Diamidino-2-phenyindole,dilactate,DAPI)标记的NSC分别与PAS、FAS共培养,10天后免疫组化法对分化神经元进行鉴定,显微镜下随机选取20个视野,每次计数100个细胞,计算NSC分化为神经元的比例。结果NSC与PAS共培养后分化成的神经元比例较高,与胶质细胞的比例约为61%:39%;FAS与NSC共培养后分化神经元数量相对较少,以胶质细胞居多,其比例约为41%∶59%。结论PAS与FAS相比更能促进NSC向神经元分化。
Objective To study the regulatory effect of protoplasmic astrocyte （PAS） and fibrous astrocyte FAS） on differentiation of neural stem cell（NSC ）. Methods Immunohistochemisty with DAPI was taken to calculate the proportion of neurons within 100 cells by 20 fields of vision chosen randomly for 10 days. The data were analyzed in statistics by co-culture of NSC with PAS and FAS respectivly. Results The percentage of neurons in PAS group （61%） was much more than that of FAS group （41%）. Conclusion The result suggested that PAS was more effective to induce NSC differentiating into neuron in vitro.
出处
《创伤外科杂志》
2007年第2期160-162,共3页
Journal of Traumatic Surgery
基金
国家自然科学基金(30300364)
重庆市院士基金(7671)
