摘要
目的 了解北京地区 1990～ 1991年和 1997～ 1998年两个非连续的流行年中呼吸道合胞病毒(RSV)A、B亚型和基因型的流行情况。方法 用间接免疫荧光法 (IIF)检测RSV阳性鼻咽分泌物 (NPS)标本或RSV分离株 ,划分A、B亚型。根据N基因片段的限制性酶切图型将RSV分离株分成至少 6个基因型NP1- 6NP1,3和 6属于B亚型 ,NP2 4和 5属于A亚型。根据SH基因片段的核苷酸序列将A亚型分离株进一步划分为SH基因型SHL1- 6。SHL之间关系密切 ,并与NP有关。SHL1,3和 4紧密相关 ,属于NP2 ;SHL2和 6属于NP4;SHL5属于NP5。结果 1997冬季～1998年春季 145份RSVNPS标本中 ,83份 (5 7 2 % )为B亚型 ,6 2份 (4 2 8% )为A亚型 ,A∶B为 1∶1 3。1997～ 1998流行年所获的 10株RSV分离株中 ,2株为A亚型 ,8株为B亚型 ,A∶B为 1∶4。 1990～1991流行年所获的 10株毒株中 ,8株为A亚型 ,2株为B亚型 ,A∶B为 4∶1。1997～ 1998流行年分离到的 10株RSV分离株中 ,2株A亚型均属NP4,SHL2 ;而 2株B亚型均为NP3。根据NP推导出的A、B亚型划分与单克隆抗体定义的亚型一致。结论 北京地区RSVA、B亚型或多个基因型可同时流行 ,但每个流行年的优势基因型可不同。相距 5年仍可分离到相同的毒株 ,表明尽管A亚型毒株之间存在明显的差异性 。
Objective To determine the epidemiologic patterns of subgroups A and B and genotypes of respriatory syncytial viruses(RSV)during two uncontinuously epidemic years from 1990 to 1991 and 1997 to 1998 in Beijing. Methods Nasopharyngeal secretion(NPS) samples of RSV positive or RSV isolates tested by indirect immunofluorescenct (IIF)assay were classified into subgroups A and B.Also, isolate of RSV were divided into at least six different lineages, designated NP1-6,by restriction mapping of the N gene. Np1,3 and 6 were given by subgroup B isolates, while NP2,4 and 5 were given by subgroup A isolates. Strains of subgroup A were further subdivided into six lineages SHL1-6 on the basis of the SH gene sequence. SH lineages were closely related to each other and to NP1-6. Strains of SHL1,3, and 4 were closely related and belonged to NP2, SHL2 and 6 to NP4 and SHL5 to NP5. Results Of 145 RSV NPS samples from the winter 1997 to the spring 1998, 83(57.2%)were subgroup B strains, 62(42.8%)were subgroup A strains, the rate of occurrence of subgroup A to B strains was about 1∶1.3. Two of 10 isolates of 1997-1998 offered by viral department of research institute of Pediatrics were subgroup A strains, whereas 8 were subgroup B strains, the rate of occurrence of subgroup A to B strains was 1∶4. Eight subgroup A strains of 10 isolates from the 1990-1991 epidemic were dominant, the proportion of subgroup A to B strains was 4∶1. of 10 RSV isolates in 1997-1998, all 2 subgroup A strains gave N gene fragment restriction pattern NP4, and fell into SH lineage SHL2, whereas 8 subgroup B strains all belonged to NP3. All 8 subgroup A isolates from the 1990-1991 epidemic gave pattern NP4, and fell into SHL2, while 2 subgroup B strains all belonged to NP3. The classification of subgroups A and B deduced from NP patterns was corresponded to the definition of these subgroups by monoclonal antibodies. Conclusions These observations confirmed that subgroups A and B or multiple lineages of RSV co-circulated in Beijng, but different genomic types predominated each year. Moreover, very similar viruses were isolated up to more than 5 years ago, indicating that despite apparent diversity of the subgroup A strains, the separate lineages might be relatively stable.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
2000年第3期274-277,共4页
Chinese Journal of Experimental and Clinical Virology
关键词
呼吸道合胞病毒
流行病学
荧光免疫测定
PCR
Respiratory syncytial virus
Genotype/Epidemiology
Fluoroimmunoassay
Polymerase chain reaction
