摘要
目的:探讨小分子干扰RNA(siRNA)靶向沉默趋化因子受体4(CXCR4)基因对卵巢癌SW626细胞增殖、迁移及侵袭的影响,为卵巢癌的基因治疗提供新的理论依据。方法:构建CXCR4干扰RNA质粒载体,重组扩增后转染CXCR4阳性的人卵巢癌细胞株SW626,以RT-PCR和Western blot检测CXCR4基因及蛋白表达的变化,MTT检测CXCR4干扰质粒对卵巢癌细胞增殖的影响,Transwell检测CXCR4干扰质粒对卵巢癌细胞迁移、侵袭能力的影响。结果:转染48h后,人卵巢癌细胞株SW626的转染效率为80%～90%,抑制了卵巢癌细胞CXCR4基因及蛋白的表达(P<0.05),部分抑制了癌细胞的增殖(P<0.01),但此作用并不随时间变化而有明显变化;癌细胞的迁移及侵袭率均明显下降,差异有显著统计学意义(P<0.01)。结论:CXCR4 siRNA可通过下调CXCR4的表达抑制人卵巢癌细胞的增殖、迁移及侵袭。
Objective：To evaluate the effect of siRNA targeting CXCR4 on the proliferation,migration and invasion of ovarian cancer cells.Methods：The CXCR4 siRNA plasmid vector was constructed and then transfected into the SW626 cells.The silence rates of CXCR4 mRNA and protein expression were detected by real time reverse transcription-polymerase chain reaction（real time RT-PCR） and Western blot respectively.The cell viability was investigated by MTT assay.The cell invasion and migration in vitro were studied by Transwell.Results：The siRNA of CXCR4 plasmid with the specific fragment was constructed successfully.The transfection efficiency of ovarian cancer cell line SW626 cells was approximately 80%-90% after transfected 48 hours.The expressions of CXCR4 mRNA and protein were significantly decreased in interference group（P0.01） compared with the control group.And the cell viability was also partially inhibited.Cell migration and invasion abilities were reduced significantly in interference group（P0.01）.Conclusion：CXCR4 siRNA can significantly inhibit the proliferation,migration and invasion of ovarian cancer cells.
出处
《现代妇产科进展》
CSCD
2012年第11期848-851,856,共5页
Progress in Obstetrics and Gynecology
基金
山东省医药卫生科技发展计划(No:2009HZ014)
