摘要
目的建立运用氨基酸自动分析仪快速测定牛乳中羟脯氨酸的方法。方法牛乳经水解,羟脯氨酸与茚三酮反应,生成黄色产物,在仪器的第二通道440 nm处有最大吸收峰,且在一定的浓度范围内符合朗伯比尔定律。以柠檬酸盐缓冲溶液为流动相,将羟脯氨酸和其他17种氨基酸同时分离,440 nm定量。结果浓度在0.05～1.0 mmol/L范围内吸收度呈良好线性关系(r=0.999 9),平均回收率为91.5%,RSD为1.3%(n=5),方法最低检出限0.66 mg/L。结论该方法快速、简便、准确,可靠,适合于牛乳中羟脯氨酸的定量分析。
Objective To establish a rapid detection method for hydroxyproline in dairy using amino acid autoanalyzer.Methods Hydroxyproline in dairy hydrolyzate could react with ninhydrin and produce a yellow compound which has an absorption peak at 440 nm in the second channel of the instrument and comply with the Lambert Beer law within a certain concentration range.Citrate buffer solution as mobile phase,hydroxyproline and other 17 kinds of amino acids were separated at 440 nm quantitatively.Results The calibration curves showed a good linarity in the range of 0.05-1.0 mmol/L（r=0.999 9）.The mean recovery was 91.5% with RSD 1.3%（n=5） and the limit of detection was 0.66 mg/L.Conclusion The method was rapid,convenient,accurate and reliable.It could be applied for quantitative detection of hydroxyproline in dairy.
出处
《中国食品卫生杂志》
北大核心
2012年第6期542-545,共4页
Chinese Journal of Food Hygiene
基金
江苏省科技支撑计划-社会发展(BE2010745)
江苏省临床医学中心(高技术平台)(ZX201109)
