摘要
依据γ-氨基丁酸（GABA）与茚三酮反应生成蓝紫色化合物,采用氨基酸自动分析仪测定,建立了保健食品中GABA的测定方法。经过对前处理条件的选择和优化,确定保健食品用0.02 mol/L HCl超声提取,用Na OH溶液调节p H至5.5,以柠檬酸盐缓冲溶液为流动相,茚三酮溶液显色,测定波长为570 nm,外标法定量,在0.5~50 mg/L范围内线性关系良好（r=0.9999）,方法检出限为0.2 mg/L,定量限为0.5 mg/L。分别在5,10和30 mg/L 3个水平进行加标和精密度实验（n=6）,回收率分别为99.2%,93.9%,94.7%,相对标准偏差分别为在5.5%,9.6%,2.2%。方法适合于保健食品中γ-氨基丁酸的定量分析。
A method for determination of γ-aminobutyric acid（GABA） in health food was established by automatic amino acid analyzer,which was used to detect a blue violet product formed by the reaction between GABA and ninhydrin. Through the selection and optimization of pretreatment conditions,the results showed that the optimum condition for extraction was as follow： the health foods were extrac ted by ultrasonic using 0. 02 mol/L hydrochloric acid as extraction solvent,sodium hydroxide solution was used to adjust p H to 5. 5,the citrate buffer solution was used as the mobile phase,ninhydrin was used as chromogenic agent,the detection wavelength was 570 nm,and the external standard method was used for quantification. GABA had a good linear relationship in the range of0. 5 ~ 50 mg/L with correlation coefficient（r） of 0. 9999. The limit of detection was 0. 2 mg/L,and the limit of quantitation was 0. 5 mg/L. The recoveries were 99. 2%,93. 9% and 94. 7%（n = 6） respectively at the spiked levels of 5,10 and 30 mg/L,and the relative standard deviations were 5. 5%,9. 6% and 2. 2%. The method is suitable for the quantitative analysis of γ-aminobutyric acid in health food.
出处
《分析试验室》
CAS
CSCD
北大核心
2016年第9期1012-1015,共4页
Chinese Journal of Analysis Laboratory
基金
江苏科技厅科技支撑计划项目(BE2010745)
江苏省"十二五"科教兴卫工程项目(ZX201109)资助
