摘要
目的建立测定门冬氨酸鸟氨酸含量的高效液相色谱(HPLC)法。方法色谱柱为Kromasil 100-5NH2柱(4.6 mm×250 mm,5μm);以乙腈-0.05 mol.L-1磷酸二氢钾溶液(50∶50)为流动相;流速为1.0 mL.min-1;检测波长为210 nm;柱温25℃;进样量为20μL。结果 3批样品中门冬氨酸鸟氨酸的平均含量为100.19%。门冬氨酸、鸟氨酸及门冬氨酸鸟氨酸在600～1 500μg.mL-1浓度范围内,都有良好的线性关系(r=0.999 7),门冬氨酸鸟氨酸的平均回收率为98.40%,RSD为0.19%。结论该方法灵敏、快速、结果准确,可用于门冬氨酸鸟氨酸的含量测定。
Objective To establish a high performance liquid chromatography （HPLC） method for the determination of ornithine aspartate injection. Methods The chromatographic separation was performed on a Kromasill00-SNH2 column （4.6 minx250 mm,5 μm）. The mobile phase consisted of 0.05 mol·L-1 potassium dihydrogen phosphate-acetonitrile （50 ： 50） ,the flow rate was 1.0 mL ·min-1 ,the detective wavelength was 210 nm,the column temperature was 25 ℃ and the sample size was 20μL. Results The average content of ornitbinaspaetant in three batches of samples was 100. 19%. Aspartate, ornithine and ornitbinaspaetant showed good linear relationships （ r = 0. 999 7 ） in the range of 600-1 500 mg· mL-1. The average recovery was 98.40% （RSD=0.19%）. Conclusion The method is sensitive,accurate,rapid and it is suitable for the assay of omithine aspaetant injection.
出处
《医药导报》
CAS
北大核心
2012年第12期1621-1623,共3页
Herald of Medicine
关键词
门冬氨酸鸟氨酸
含量测定
色谱法
高效液相
Ornithine aspartate injection
Content determination
High performance liquid chromatography
