摘要
目的:采用蛋白质抗体芯片技术观察金黄扶正散对免疫抑制小鼠细胞因子的影响。方法:皮下注射环磷酰胺建立免疫抑制小鼠模型,金黄扶正散低、中、高剂量组分别连续给小鼠灌胃10 d,末次用药后小鼠禁食12 h,各组小鼠尾静脉取血1 mL,离心,取血清备用,采用细胞因子抗体芯片进行细胞因子定量检测。结果:与对照组比较,环磷酰胺组的2种细胞因子IFN-γ和RANTES呈显著性降低(P<0.05),金黄扶正散低、中、高剂量组给药后IFN-γ有显著性提升,高剂量组的RANTES呈显著性升高(P<0.05);与对照组比较,环磷酰胺组的5种细胞因子IL-5,IL-6,IL-9,IL-13和MCP-1呈显著性升高(P<0.01,P<0.05),给药组给药后有不同程度降低(P<0.01,P<0.05);13种细胞因子GM-CSF,IL-1α,IL-1β,IL-2,IL-3,IL-4,IL-10,IL-12,IL-17,M-CSF,TNF-α,KC和VEGF无明显变化。结论:金黄扶正散对免疫抑制小鼠20种细胞因子有不同程度的影响,这可能与调节免疫抑制小鼠Th1/Th2亚群有关。
Objective： To observe the effect of Jinhuang Fuzheng powder on cytokines of immunosuppressive mice by using protein antibody micro-array. Method： The immunosuppressive mice model was established by subcutaneously injecting cyclophosphamide. Rats were orally administered with low, middle and high dose of Huangjin Fuzheng powder for 10 days, and fasted for 12 hours after the final administration. 1 mL blood was drawn from caudal veins and isolated hearts of rats of each group. A quantitative test was conducted for cytokines with cytokine antibody array. Result： Compared with the control group, IFN-T and RANTES of the CTX group decreased significantly （P 〈0. 05）. After the administration, IFN-T of low, middle and high-dose groups, and RANTES of the high-dose group increased significantly （P 〈0. 05）. Compared with the control group, IL-5, IL-6, IL-9, IL-13 and MCP-1 of the CTX group increased remarkably （P 〈0. 01 ,P 〈0. 05）. After the administration, IL-5, IL-6, IL-9, IL-13 and MCP-1 of low, middle and high-dose groups de- creased to varying degrees. GM-CSF, IL-la, IL-1/3, IL-2, IL-3, IL-4, IL-10, IL-12, IL-17, M-CSF, TNF-a, KC and VEGF of the 13 types of cytokines showed no significant change. Conclusion： Jinhuang Fuzheng powder shows effect on 20 types of cytokines of immunosuppressive mice to varying degrees, which may be related to the regulatory immunosuppression of Thl/Th2 subgroup in mice.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2012年第23期3624-3627,共4页
China Journal of Chinese Materia Medica
基金
广西中医药管理局项目(GZYZ-10-31)
广西教育厅项目(201010LX051)
关键词
金黄扶正散
免疫抑制
蛋白质抗体芯片
Jinhuang Fuzheng powder
immunosuppression
protein antibody micro-array
