摘要
目的 探讨人白细胞 DR抗原 (HL A- DR)在人原发性肝细胞癌 (简称肝癌 )的表达情况 ,以及干扰素 (IFN)对其调节作用 .方法 分别采用免疫组化 ABC法和流式细胞仪检测 HL A- DR在 46例人肝癌组织和 4种培养的人肝癌细胞系 (SMMC- 772 1,HCC- 92 0 4,BEL- 740 2和 HHCC)的表达情况 ,并用 EL ISA法分别检测 IFN-γ或 IFN-α刺激前后 HL A-DR在上述 4种肝癌细胞系以及正常人肝细胞系 QZG的表达情况 .结果 39.1% (18例 / 46例 )的肝癌组织肿瘤细胞可表达 HL A- DR,而在癌旁非肿瘤细胞均为阴性 ;4种肝癌细胞系都基本不表达 HL A- DR;IFN- γ或 IFN- α刺激之后 ,上述 5种细胞系的 HL A- DR表达都有所增强 ,并且 QZG不如肝癌细胞明显 ,IFN-γ的作用强于 IFN-α.结论 肝癌组织在一定程度上可表达 HL A- DR,但是培养的肝癌细胞系基本上不表达 ;IFN可以上调肝癌细胞对 HL A- DR的表达 .
AIM To study the expression of human leukocytic antigen DR (HLA DR) in primary human hepatocellular carcinoma (HCC) and the boosting effect of interferon (IFN) on it. METHODS The expression of HLA DR in 46 human HCC tissues and 4 cultured human HCC cell lines (SMMC 7721, HCC 9204, BEL 7402 and HHCC) was detected with immunohistochemical ABC method and flow cytometer, respectively. The expression of HLA DR in the above 4 HCC cell lines and a normal human liver cell line QZG was detected with ELISA method before and after being stimulated by IFN γ or IFN α, respectively. RESULTS The tumor cells of 39.1% (18 cases/ 46 cases) of the HCC tissues expressed HLA DR, while all the normal liver cells immediately adjacent to HCC were HLA DR negative. On the whole, HLA DR was negative in all of the 4 kinds of HCC cell lines. The expression of HLA DR was enhanced in all of the above 5 cell lines after being stimulated by IFN γ or IFN α. It was less obvious in QZG than in HCC cells. The effect of IFN γ was more obvious than that of IFN α. CONCLUSION The HCC tissues can express HLA DR to some extent. On the whole, the cultured HCC cell lines do not express HLA DR. IFN can enhance the expression of HLA DR in HCC cells.
出处
《第四军医大学学报》
2000年第3期283-285,共3页
Journal of the Fourth Military Medical University
基金
国家863课题!(102-09-01-02)
关键词
HLA-DR
肝细胞癌
干扰素
免疫组织化学
ELISA
human leukocytic antigen DR
hepatocellular carcinoma
interferon
immunohistochemistry
flow cytometer
ELISA
