摘要
目的：探讨干扰素（IFN）对肝癌细胞HLA－DR表达的调节作用。方法：采用免疫组化ABC法和ELISA法分别检测了不同剂量的IFN－γ或IFN－α刺激前后HLA－DR在HHCC、SMMC－7721、BEL－7402和HCC－9204等4种人肝细胞癌细胞系以及正常人肝细胞系QZG的表达情况。结果：IFN－γ或IFN－α刺激之前，5种细胞系中只有少数的SMMC－7721细胞可表达HLA－DR；刺激之后，对HLA－DR的表达5种细胞系都有所增强，并且与IFN－γ的剂量呈正相关，QZG不如肝癌细胞明显，IFN－γ的作用强于IFN－α。结论：IFN可以上调肝癌细胞对HLA－DR的表达。关键词：##4干扰素;;HLA－DR;;肝细胞癌;;免疫组织化学;;
Object: To require into the regulative effect of interferon (IFN) on HLA－DR expression in human heaptocellular carcinoma (HCC). Methods: The expression of HLA－DR in 4 HCC cell lines, HHCC, SMMC－7721, BEL－7402,HCC－9204 and a normal liver cell line QZG was detected with immunohistochemical ABC and ELISA methods before and after stimulated by different doses of IFN－γ or IFN－α. ResultS: Only a little part of SMMC－7721 cells expressed HLA－DR before stimulation by IFN－γ or IFN－α, and the others were HLA－DR negative; HLA－DR expression enhanced in all of the 5 cell lines after stimulation, and was correlated with the dose of IFN, QZG was less obvious than HCC cells, the effect of IFN－γ was obvious than IFN－α. Conclusion: IFN can induce HLA－DR expression in HCC cells and make self－immunological adjustment in HCC on some extent.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
1999年第2期139-141,共3页
Chinese Journal of Cancer
基金
国家863课题!10-09-01-02
国家自然科学基金!39770827
