摘要
以魔芋球茎为外植体,研究组织培养过程中有效降低外植体褐变的方法。采用MS+6-BA1mg/L+NAA1mg/L为基本培养基,用正交试验方法研究不同琼脂浓度、吸附剂及光、暗2种不同培养条件下,对魔芋组织培养过程中褐变的影响。在MS+6-BA1mg/L+NAA1mg/L+蔗糖30g/L+琼脂6g/L+AC0.5g/L+PVP0.05g/L及暗培养条件下,魔芋外植体的褐变率减低为38.3%,愈伤组织诱导率提高到78.3%。在培养基中加入一定量的活性炭(AC)和聚乙烯吡咯烷酮(PVP),并进行暗培养,可在一定程度上控制魔芋外植体的褐变,促进愈伤组织形成。
Using Amorphophallus konjac bulbs as explants,the research studied method of reducing browning of explants in tissue culture process.By using MS＋6-BA 1 mg/L＋ NAA 1 mg/L as the basic medium and the orthogonal experimental method to study the impact on browning with different agar concentrations,adsorbent and light,dark two kinds of different culture conditions.The results showed that the callus growth speed was enhanced and the browning of explants was under control in the combination of MS ＋ 6-BA 1 mg/L ＋ NAA 1 mg/L＋ sugar 30 g/L＋ agar 6 g/L＋AC 0.5 g/L＋PVP 0.05 g/L.It can make to reduce the browning rate of 38.3%,callus induction rate increased to 78.3%.The konjac bulbs explants the browning can be effectively controlled,which add a certain amount of activated carbon（AC） and polyvinylpyrrolidone（PVP） in the culture medium and in the dark conditions,its callus can be promptly formed.
出处
《中国农学通报》
CSCD
2012年第31期243-246,共4页
Chinese Agricultural Science Bulletin
基金
安康学院大学生科技创新项目"降低魔芋组培苗褐变的研究"(2010AKXYDXS26)
安康学院项目"魔芋新品种组培快繁技术研究"(2008AKXY016)
陕西省科技厅农业攻关项目"秦巴山区野菜引种驯化和魔芋新品种选育研究与示范"(2010K01-23)
关键词
魔芋
组织培养
褐变
正交试验
Amorphophallus konjac
tissue culture
browning
orthogonal experiment
