摘要
采用不同激素配比的液体和固体培养基对花魔芋进行了组织培养研究。结果表明:浅层液体培养基在花魔芋组织培养时较固体培养基具有褐变率低、愈伤组织生长速度快、平均出芽数高、根粗壮、成本低等优势;初代培养基应选择MS+6-BA0.5mg·L-1+NAA0.5mg·L-1+蔗糖3.0%+0.1%PVP(pH5.8),出芽培养基应选择MS+6-BA2.0mg·L-1+NAA0.5mg·L-1+蔗糖3.0%+0.1%PVP(pH5.8),待出芽后转入MS+6-BA1.0mg·L-1+NAA0.5mg·L-1+蔗糖3.0%+0.1%PVP(pH5.8),二者交替培养可连续批量获取组培苗,生根培养基应选择1/2MS+IAA0.5mg·L-1+蔗糖1.5%+0.1%PVP(pH5.8)。花魔芋愈伤组织继代3次后发现了A型、B型、C型3种愈伤组织,三者在外观、组织结构和分化能力上存在差异,试验和生产上选择A型和B型愈伤组织进行分化培养可得到高效稳定的分化体系。
Amorphophallus konjac K.Koch tissue culture was studied in liquid and solid MS medium supplemented with different hormone proportion.The result showed that shallow liquid medium was prior to solid medium in browning control,callus growth speed,number of differentiation buds,robustness of roots and low cost.MS+6-BA 0.5 mg·L-1+NAA 0.5 mg·L-1+Sucrose 3.0 %+0.1 % PVP(pH 5.8)could be used for primary culture.Alternative culture in MS+6-BA 2.0 mg·L-1+NAA 0.5 mg·L-1+Sucrose 3.0 %+0.1 % PVP(pH 5.8)and MS+6-BA 1.0 mg·L-1+NAA 0.5 mg·L-1+Sucrose 3.0 %+0.1 % PVP(pH 5.8)could produce tissue culture plantlets in batch and continuously.Rooting medium could be 1/2MS+IAA 0.5 mg·L-1+Sucrose 1.5 %+0.1 % PVP(pH 5.8).3 types of calli were found after subculture for 3 times.Remarkable difference existed in the A,B and C types in appearance,organization structure and capacities of re-differentiation.Calli of A and B-type were chosen for bud differentiation,both in test and application,which could produce a stable and efficient differentiation system.
出处
《中国蔬菜》
北大核心
2011年第10期58-63,共6页
China Vegetables
基金
重庆市科委攻关项目(CSTC.2010AC1013)
中央高校基本科研业务费专项资金资助项目(XDJK2009B022)
关键词
魔芋
浅层液体培养
褐变
愈伤组织
Amorphophallus konjac K. Koch
Shallow liquid tissue culture
Browning
Callus
