摘要
以不同类型的白魔芋Md和Ms与花魔芋CMH和CCDY4种材料的块茎为外植体,培养于附加6-BA和NAA不同浓度组合的MS培养基上进行愈伤组织诱导、不定芽分化及试管苗生根寄栽等试验。结果表明,培养效果与魔芋的类型无关,主要与培养基中激素的浓度组合相关。培养的适宜激素浓度组合,诱导带芽愈伤组织CMH和Md为6-BA1.0mg/L+NAA0.5mg/L,CCDY和Ms为6-BA1.0mg/L+NAA1.0mg/L;不定芽分化及快繁,花魔芋为6-BA1.0mg/L+NAA0.5mg/L,白魔芋为6-BA2.0mg/L+NAA0.5mg/L。试管苗生根以NAA0.5mg/L+IBA0.1mg/L激素浓度组合较好。愈伤组织诱导及不定芽分化应在黑暗或散射光下培养,可大大减少切块伤口褐化程度;愈伤组织继代以10mm×10mm×10mm大小及不定芽增殖以带3个小芽的组织切块转接,利于快速生长。加入多酚氧化酶抑制剂(Vc、柠檬酸)及吸附剂(活性碳)对抑制褐化效果并不明显。
The tubers of two genotypes of Amorphophallus albus Liu et Chen (Md and Ms) and two genotypes of A. konjac K. Koch (CMH and CCDY) were used as explants and cultured on MS media supplemented with different combinations of 6-BA and NAA for callus induction, adventitious bud differentiation and plantlet rooting. The culture effect seemed independent of the species of konjac and was mainly related to the combination of plant hormones. The optimum combination of hormones for callus induction was BA 1.0 mg/L + NAA 0.5 mg/L for CMH and Md and BA 1.0 mg/L + NAA 1.0 mg/L for CDDY and Ms. The optimum hormone combination for adventitious bud differentiation and rapid propagation was BA 1.0 mg/L + NAA 0.5 mg/L for A. konjac and BA 2.0 mg/L + NAA 0.5 mg/L for A. albus The combination NAA 0.5 mg/L + IBA 0.1 mg/L gave satisfactory results of plantlet rooting. Callus induction and adventitious bud differentiation carried out in the dark or under disperse light greatly reduced browning of the explants. Callus tubes of 10 mm x 10 mm x 10 mm gave desirable results in subculture and tissues with three adventitious buds were satisfactory explants for rapid multiplication. Addition of vitamin C or citric acid (polyphenol oxidase inhibitors) or the adsorbent active carbon gave poor results for inhibiting browning.
出处
《西南农业大学学报（自然科学版）》
CSCD
北大核心
2003年第4期309-312,共4页
Journal of Southwest Agricultural University
基金
重庆市科委攻关项目(2000-6343)
关键词
魔芋
类型
组织培养
褐化
快速繁殖
Amorphophallus
type
tissue culture
browning
rapid propagation
