摘要
建立了高效液相色谱同时测量软饮料中20种食品添加剂(咖啡因、3种甜味剂、9种防腐剂和7种着色剂)的方法。确定的最佳分离条件为:C_(18)反相色谱柱(250 mm×4.6 mm×5μm),流动相为0.10 mol/L乙酸铵缓冲溶液(pH 7.2)和乙腈-甲醇混合有机相(10:90,V/V),梯度洗脱,二极管阵列检测器在190～700 nm监测。20种食品添加剂浓度在0.10～300 mg/L范围内具有良好的线性,相关系数大于0.998;20种食品添加剂的检出限在0.005～0.110 mg/L范围内;20种食品添加剂的加标回收率为91.4%～100.5%;相对标准偏差计为0.6%～4.0%。本方法可应用于16个品牌的软饮料中食品添加剂的检测。
An efficient and accurate analytical method was developed for the simultaneous determination of 20 synthetic food additives,including acesulfame potassium,benzoic acid,sorbic acid,sodium saccharin,tartrazine,amaranthus red,ponceau 4R,caffeine,sunset yellow,aspartame,allure red, brilliant blue,erythrosine,methyl paraben,ethyl paraben,isopropyl paraben,n-propyl paraben, isobutyl paraben,n-butyl paraben and n-heptyl paraben,by high performance liquid chromatography （HPLC） coupled with photodiode array detector（PDA）.A C_（18） stationary phase was used and the mobile phase contained an acetonitrile-methanol（10：90,V/V） mixture and 0.1 mol/L ammonium acetate buffer solution at pH 7.2.Successful separation was obtained for all the compounds within 50 min at 30℃by using gradient elution.The absorbance of 20 compounds behaved linearly with their concentrations ranged from 0.10 mg/L to 300 mg/L with correlation coefficients more than 0.998.The limits of detection of these compounds were from 0.005 mg/L to 0.110 mg/L.The recoveries were from 91.4%to 100.5%at spiked levels of 10—100 mg/L,The precision（n = 6） was range from 0.6%to 4.0%.The method has been used in the determination of 16 food additives in various drinks.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2012年第11期1661-1667,共7页
Chinese Journal of Analytical Chemistry
基金
国家质检总局食品安全专项(No.ASPAQ1010)
中国计量科学研究院基础科研费(No.AKY1216)资助
关键词
食品添加剂
高效液相色谱
二极管阵列检测器
同时测定
软饮料
Food additives
Liquid chromatography
Photodiode array detection
Simultaneous determination
Drinks
