摘要
目的观察左归饮对围绝经期大鼠卵巢一氧化氮(nitric oxide,NO)生成的影响及机制。方法对围绝经期大鼠动物模型,分别给予低、中、高剂量左归饮及尼尔雌醇灌胃8周,模型组及青年对照组(对照组)予生理盐水灌胃。用硝酸酶还原法、化学比色法分别检测卵巢NO含量、总一氧化氮合酶(nitric oxide synthase,NOS)活性;用RT-PCR和免疫组化法检测卵巢诱导型NOS(inducible NOS,iNOS)、内皮型NOS(en-dothelial NOS,eNOS)及神经型NOS(neuronal NOS,nNOS)mRNA及蛋白的表达。结果 (1)模型组大鼠卵巢NO含量、总NOS活性显著低于对照组(P<0.01),中、高剂量左归饮可使其明显增高(P<0.01,P<0.05);(2)模型组大鼠卵巢中eNOS、iNOS、nNOS表达均明显低于对照组(P<0.01),中剂量左归饮可显著促进初老大鼠卵巢此3种NOS表达(P<0.01),高剂量可使iNOS、eNOS表达增高(P<0.01),而低剂量仅使iNOS表达增强(P<0.01)。结论卵巢局部eNOS、iNOS、nNOS表达的下调,导致的NOS活性降低、NO生成减少,均与围绝经期大鼠卵巢微循环血管功能受损密切相关;左归饮可通过上调eNOS、iNOS、nNOS表达,增加围绝经期大鼠卵巢NOS活性、NO生成,对衰老卵巢微循环发挥保护作用。
Objective To study the effects and mechanisms of Zuoguiyin （ZGY） on the ovarian nitric oxide （NO） production in peri-menopausal rats. Methods The peri-menopausal model rats were respectively administered with low （13.78 g/kg）, middle （20.67 g/kg）, and high （31.00 g/kg） dose ZGY, and nilestriol for 8 weeks. Normal saline was given by gastrogavage to rats in the model group and the young control group （as the control group）. The ovarian NO content and the activity of total nitric oxide synthase （NOS） were detected using nitrate reductase method and chemical colorimetry respectively. The mRNA and protein expressions of inducible NOS （iNOS）, endothelial NOS （eNOS）, and neuronal NOS （nNOS） were detected using RT-PCR and immunohistochemical assay. Results （1） Compared with that in the control group, the ovarian NO content and the activity of total NOS in peri-menopausal rats were significantly lower （P0.01）. Middle and high dose ZGY could obviously up-regulate them （P0.01, P0.05）. （2） The three kinds of NOS expression levels in peri-menopausal rats were obviously lower when compared with those of the control group （P0.01）. Middle dose ZGY could significantly promote all the three kinds of NOS expression levels of pre-senile rats （P0.01）. High dose ZGY could up-regulate the expressions of iNOS and eNOS, while low dose ZGY could only enhance the iNOS expression （P0.01）. Conclusions The down-regulated expressions of eNOS, iNOS, and nNOS in local ovaries resulted in decreased NOS activity and NO production, which were closely correlated with damaged microcirculatory vascular functions of ovaries in peri-menopausal rats. ZGY could protect rats′ ovarian microcirculation by up-regulating the expressions of eNOS, iNOS, and nNOS, and enhancing the ovarian NOS activity and NO production.
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2012年第11期1549-1553,共5页
Chinese Journal of Integrated Traditional and Western Medicine
基金
黑龙江省教育厅科学技术研究项目(No.12511530)
