摘要
目的:探讨左归饮及其部分组方对老年小鼠细胞免疫功能的作用。方法:将ICR健康雄性老年小鼠随机分为老年对照组(Old contrast group,OC)、左归饮组(Zuoguiyin group,ZGY)、单味熟地组(Prepared rhizome of rehmannia group,PROR)、去熟地组(None prepared rhizome of rehmannia group,NPROR)等4组。常规水煎左归饮及其部分组方,配成100g/L浓度的药水混合液,供中药各组小鼠自由饮用75天,老年对照组小鼠饮用自来水。(1)测胸腺指数,脾指数;(2)用MTT法测定脾脏T淋巴细胞增殖活性;(3)用酶联免疫吸附(ELISA)法测定血清中IL-2水平。结果:ZGY可增强老年小鼠特异性细胞免疫功能,显著提高老年小鼠的胸腺指数和脾指数,增强T淋巴细胞活性,同时增加体内IL-2表达水平,而单味熟地仅能显著增强老年小鼠T淋巴细胞活性,对其它免疫衰老指标影响不大,去熟地组对老年小鼠的细胞免疫功能影响不显著,显示出整方抗衰老的优势。结论:左归饮可能是通过提高细胞免疫功能来发挥其抗衰老作用。
Objective：To explore the effect of zuoguiyin and its partial prescriptions on cellular immune function in senile mice. Methods：Healthy male ICR senile mice were divided into 4 groups at random： old contrast group, ZGY group, prepared rhizome of rehmannia（PROR） group and none prepared rhizome of rehmannia（NPROR） group. The senile mice had been drunk zuoguiyin and its partial prescriptions for 75 days, then observing the differences through thymic index, spleen index, activity of T lymphocyte and the level of IL-2 after using different drugs. Results：Acted by ZGY, ZGY had enhanced cellular immune function of senile mice, appearing that thymic index, spleen index, activity of T lymphocyte and the level of IL-2 were distinctly higher than those of the old contrast group. Compared to ZGY, prepared rhizome of rehmannia also enhanced the activity of T lymphocyte. However, it had no effect on thymic index, spleen index and the level of IL-2. So its effect on anti-senility was not better than ZGY. None prepared rhizome of rehmannia group had no distinct effect on those indexes. Conclusion：Zuoguiyin had an action of antisenility by improving cellular immune function.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2006年第9期842-844,共3页
Chinese Journal of Immunology
基金
皖南医学院中青年科研基金项目(WK2002148)资助
关键词
左归饮
熟地
衰老
细胞免疫
IL-2
Zuoguiyin
Prepared rhizome of rehmannia
Senility
Cellular immune
Interleukin-2
