摘要
背景:补肾法正逐步成为中医老年病学中抗衰老的治疗方法之一。目的:探讨滋补肾阴方左归饮对老年小鼠自由基代谢血清丙二醛含量,超氧化物歧化酶活性的影响。设计:完全随机对照实验。单位:广东医学院微生物与免疫学教研室。对象:实验于2002-09/2004-07在皖南医学院免疫学研究室完成,选择四川省医学科学院实验动物研究所提供的40只雄性老年封闭群小鼠。随机分为老年对照组、左归饮组、单味熟地组及去熟地组,每组10只。左归饮由熟地、山药、枸杞、炙甘草、茯苓、山茱萸,按9:6:6:3:4:5比例组成。常规水煎,左归饮组浓缩至含生药0.5775g/mL,单味熟地组浓缩至含生药0.1575g/mL,去熟地组浓缩至含生药0.42g/mL,配成100g/L浓度的药水混合液。干预:左归饮组、单味熟地组、去熟地组饮用左归饮、熟地、去熟地,4次/d,1.5mL/次,共75d,给药期间不提供其他饮用水。老年对照组小鼠饮用自来水。饮用75d后,摘眼球取血2mL,留存血清,用硫代巴比妥酸法测定血清中丙二醛含量,黄嘌呤氧化酶法测定血清中超氧化物歧化酶活性,比色法测定血清总抗氧化能力。主要观察指标:①血清中丙二醛含量。②血清中超氧化物歧化酶活性。③血清总抗氧化能力检测。结果:老年对照组死亡1只,单味熟地组死亡3只,最终36只小鼠进入结果分析。①血清中丙二醛含量:左归饮组和单味熟地组低于老年对照组[(5.280±1.204)μmol/L和(5.886±2.717)μmol/L,(9.533±3.494)μmol/L,(q=3.641～4.657,P<0.05)]。左归饮组和单味熟地组老年小鼠血清中丙二醛含量基本一致。②超氧化物歧化酶活性:左归饮组和去熟地组高于老年对照组[(7.008±0.782)mkat/L和(6.989±0.809)mkat/L,(5.908±0.392)mkat/L,(q=3.641～4.657,P<0.05)],左归饮组和去熟地组超氧化物歧化酶活性基本一致。单味熟地组与老年对照组超氧化物歧化酶活性基本一致。③总抗氧化能力:左归饮组和单味熟地组虽然高于老年对照组,去熟地组低于老年对照组,但差异均无显著性意义(P>0.05)。结论:左归饮能明显降低老年小鼠血清中丙二醛含量,并能提高血清中超氧化物歧化酶活性,表明左归饮在改善老年机体自由基代谢、增强超氧化物歧化酶活性和减少丙二醛积蓄对机体损伤方面具有明显作用。
BACKGROUND: The method of replenishing kidney has becoming one of the therapies for anti-senility in gerontology in Chinese medicine. OBJECTIVE: To probe into the effects of the formula for nourishing kidney yin-zuoguiyin on serum malondialdehyde (MAD) content and superoxide dismutase (SOD) activity in metabolism of free radical. DESIGN: Complete randomized control experiment. SETTINGS: Microbiology and Immunology Department of Guangdong Medical College. PARTICIPANTS: The experiment was performed in Immunology Research Room of Wannan Medical College from September 2002 to July 2004. Forty male senile ICR mice were employed, provided by Institute of Experimental Animal of Medical Scientific Academy in Sichuan. They were randomized into senile control, zuoguiyin group, single shudi (Radix Rehmanniae Praeparata) group (single group) and shudi (Radix Rehmanniae Praeparata) removal group (removal group), 10 mice in each one. Zuoguiyin composes of shudi (Radix Rehmanniae Praeparata), shanyao (Rhizoma Dioscoreae), gouqi (Fructus Lycii), zhi gancao (Radix Glycyrrhizae Praeparata), fuling (Poria) and shangyurou (Fructus Corni) at the ratio of 9:6:6:3:4:5. After decocted routinely, the solution in zuoguiyin group was concentrated to 0.577 5 g/mL of raw herbs contained; that in single group was to 0.157 5 g/mL and that in removal group was to 0.42 g/mL. The mixed medical solutions of 100 g/L were prepared successively in each group. INTERVENTIONS: In zuoguiyin group, single group and removal group, solutions of zuoguiyin and shudi (Radix Rehmanniae Praeparata) and solution with shudi (Radix Rehmanniae Praeparata) removed were administrated successively, 4 times/day, 1.5 mL/time, totally for 75 days. No any drink was provided during the medication. The mice in senile control drank pipe water. After the eyeball extracted, blood of 2 mL collected and serum preserved, the thiobarbituric acid method was used to determine MAD content, the xanthine oxidase method was to determine SOD activity and the colorimetric method was to determine general capacity of anti-oxidation in serum. MAIN OUTCOME MEASURES: ① serum MAD content; ② serum SOD activity; ③ general capacity of anti-oxidation in serum. RESULTS: One mouse was dead in senile control and 3 mice in single group. Terminally, 36 mice entered result analysis. ① MAD content: Serum MAD content in zuoguiyin group and single group were lower than that in senile control [(5.280±1.204) μmol/L and (5.886±2.717) μmol/L, (9.533±3.494) μmol/L, (q=3.641-4.657, P < 0.05)]. Serum MAD content in zuoguiyin group and single group were basically same. ② SOD activity: SOD activity in zuoguiyin group and removal group were higher than senile group [(7.008±0.782) mkat/L and (6.989±0.809) mkat/L, (5.908±0.392) mkat/L, (q=3.641-4.657, P <0.05)]. SOD activity in zuoguiyin group and removal group were basically same and that in single group and senile control were basically same. ③ General anti-oxidation capacity: The general capacity of anti-oxidation in serum in zuoguiyin group and single group were stronger than that in senile control, that in removal group was weaker than senile control, but the differences were not significant (P > 0.05). CONCLUSION: Zuoguiyin remarkably reduces MAD content and improves SOD activity in serum of senile mice. It is indicated that zuoguiyin acts remarkably on improving metabolism of free radical in senile body, strengthening SOD activity and reducing organic injuries caused by MAD accumulation.
出处
《中国临床康复》
CSCD
北大核心
2005年第23期218-219,共2页
Chinese Journal of Clinical Rehabilitation
基金
皖南医学院中青年科研基金项目(W K2002148)~~
