摘要
目的 研究肺灌洗及灌洗次数对早期矽肺鼠呼吸功能的影响。方法 30只Wistar大鼠随机分为A、B、C三组 ,每组 10只。A、B两组经气管内注入二氧化硅染尘 ,C组经气管内注入等量的生理盐水。 15d后 ,A组接受全肺灌洗 10次 ,B组 2 0次 ,C组与A组同。通过动脉血气分析 ,潮气量 ,肺压力 容量值以及肺灌洗液中蛋白质和磷脂的浓度评价呼吸功能。结果 肺灌洗前A、B组的PaO2 明显低于C组。灌洗后A组的PaO2 有增高的趋势 ,PaCO2 无明显改变。B、C组PaO2 进行性降低 ,PaCO2 明显增高 ,以C组变化明显。灌洗前A、B组潮气量明显低于C组 ,灌洗后A组无明显变化 ,B、C组动态顺应性及静态顺应性明显降低。灌洗液中A、B组蛋白质浓度高于C组 ,磷脂浓度无明显差异。结论 肺灌洗能改善早期矽肺鼠呼吸功能 。
Objective To investigate the effects of lung lavage on respiratory function of experimental silicosis.Methods Thirty rats were randomly divided into three group: A, B and C. An intratracheal injection of 50 mg silica in 1 ml of sterile saline was given in group A and B, and in group C the same amount of normal saline was injected into trachea following the anesthesia with ether. Fifteen days later, the animals were anesthetized with an intraperitoneal injection of pentobarbital sodium 30 mg/kg. After endotracheal intubation through a trachotomy, three animals were connected in parallel to a pressure controlled ventilator. The respiratory frequency was set at 28 bpm with a 50% inspiration time, and 100% oxygen was used as the inspiratory gas. Throughout the experiment, the peak inspiratory pressure (PIP) was fixed at 2.45 kPa (25 cm H 2O) and the end expiratory pressure (PEEP) at 0.69 kPa (7.0 cm H 2O). Group A and C was lavaged 10 times respectively and group B was lavaged 20 times. Arterial blood gases, tidal volume, pressure volume and lung lavage fluid constituents were assessed. Results Arterial partial pressure of oxygen (PaO 2 ) in group C was higher than that in group A and B before lavage, increased significantly in group A and decreased significantly in group B and C after lavage, but arterial partial pressure of carbon dioxide (PaCO 2 ) rose gradually in group B and C . The vary amount of PaO 2 and PaCO 2 of group C was more significant than of group B. Tidal volume in group C was higher than that in group A and B before lavage, decreased significantly after lavage in group B and C. There were significant differences among three groups in pressure volume ratio. Protein concentrations in lung lavage fluid of group A and B were much higher than of group C, but phospholipid concentrations in lung lavage fluid had no different.Conclusions Lung lavage can improve respiratory function of early experimental silicosis. Unsuitable lavage may cause respiratory dysfunction.
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2000年第5期298-300,共3页
Chinese Journal of Anesthesiology
关键词
支气管肺泡灌洗
矽肺
呼吸功能试验
Brochoalvolar lavage
Silicosis
Respiratory function tests
