摘要
单核苷酸多态性(SNP)标记具有二态性等优点,可用于种子纯度鉴定。应用焦磷酸测序技术对黄瓜SNP位点CLA13(C/G)进行了分析。结果表明,该位点在16个黄瓜杂交种中多态信息量达0.556,处于高度多态,适于其中7个品种的纯度检测;为提高种子纯度鉴定效率,引入DNA Pooling技术,建立高效率的3Pooling SNP Pyrosequencing检测分析模型,并验证了分析模型的可行性。
Single nucleotide polymorphism（SNP） can be used for identification of seed purity because its advantages of density,genetic stability and bi-allelic maker.The SNP site CLA13（C/G） of 16 cucumber varieties was analyzed by Pyrosequencing.The results showed that the polymorphism information content（PIC） of this site in all of 16 hybrid cucumber varieties is 0.556,which is high polymorphism,and it is suitable for identification of seed purity for seven cucumber varieties.In order to improve the efficiency for identification of seed purity,the DNA pooling technology was used,and a high efficient 3Pooling SNP Pyrosequencing molecular assay model was established.Moreover,the feasibility of the assay model was verified.
出处
《湖南农业科学》
2012年第10期9-11,14,共4页
Hunan Agricultural Sciences
基金
天津市科技支撑项目(10ZCGYNC00400)
天津市应用基础及前沿技术研究计划(10JCYBJC09300)
