摘要
黄瓜的分子标记连锁图谱研究已经到了比较和整合的阶段,然而可用于黄瓜作图的锚定标记却并不多。本研究利用华北类型和欧洲温室型黄瓜自交系S94和S06作为PCR模板,通过将二者差异的RAPD和SRAP条带克隆、测序并根据测序结果设计特异引物,成功获得了118个SCAR标记。经4个典型黄瓜种质材料PCR验证,引物多态性比例高达10%以上。本研究获得的SCAR可望用于黄瓜遗传图谱的构建和整合。
It would be the time for integration of molecular linkage maps in cucumber, but there is little of the anchor markers for using map integration. In this study, North China type inbred line S94 and Europe Greenhouse type inbred line S06 were used as PCR DNA template denotes. Totally 118 SCAR markers have been obtained from special primers designed by the sequences of RAPD and SRAP polymorphic bands between S94 and S06. These SCAR markers obtained in this research could be at least 10% polymorphic primers validated among the four typical cucumber germplasms, which would be used to constructing the molecular linkage map in cucumber.
出处
《分子植物育种》
CAS
CSCD
2007年第3期393-402,共10页
Molecular Plant Breeding
基金
上海市自然科学基金项目(05ZR14070)
上海市科委基础研究项目(06JC14035)资助。
