摘要
为了探讨黑素皮质素受体4(melanoeortin receptor-4,MC4R)基因多态性对罗威纳杂交犬生长性状的影响,以罗威纳杂交犬混合DNA为模板,对MC4R基因进行PCR扩增,扩增产物直接测序筛选多态性位点。结果发现3个碱基颠换,分别是154 bp处T/C、755 bp处G/T及895 bp处T/C颠换,其中895 bp处T/C颠换存在于ApaⅠ酶切位点内,以此建立了基于ApaⅠ的PCR-RFLP检测方法,并对67只罗威纳杂交犬进行检测分析。结果表明,在受检罗威纳杂交犬中检测到AA(0.104)、AB(0.493)和BB(0.403)3种基因型,AB基因型体重显著高于BB基因型,AA、AB基因型胸围极显著高于BB基因型。可以考虑将MC4R基因作为犬体重、体尺性状选择的候选基因。
To investigate the influence of MC4R gene polymorphism on the growth trait of Rottweiler crossbred dog,MC4R gene were amplified base on DNA pools of Rottweiler crossbred dogs,and the PCR production was screened potential single nucleotide polymorphisms（SNPs） by sequencing.Three conversions were detected,T154C,G755T and T895C.The PCR-RFLP method was established to assay the T/C conversion mutation at base position 895 site of MC4R Gene.The results showed that three genotypes of AA,AB and BB were detected in 67 dogs,the frequencies were 0.104,0.493 and 0.403,respectively.The body weight of AB genotype was significantly higher than that of BB genotype,especially the chest circumference of AA and AB genotypes were significantly higher than that of BB genotype.MC4R gene could be a candidate gene for canine body weight and body size.
出处
《中国畜牧兽医》
CAS
北大核心
2011年第5期126-130,共5页
China Animal Husbandry & Veterinary Medicine
基金
江苏省高技术研究计划(BG2007326)
