摘要
背景:苏木精-伊红染色和Masson染色是常用的两种评价骨组织修复程度的方法,但上述两种方法能否有效地评价骨缺损修复过程中骨组织成熟度,目前尚缺乏相关研究。目的:比较Masson染色和苏木精-伊红染色两种方法判定骨组织成熟度的敏感性。方法:构建新西兰白兔15mm桡骨缺损的动物模型,植入丝素蛋白/羟基磷灰石/骨髓基质干细胞的组织工程化骨,分别于术后第4,8,12周随机选取6只动物摄X射线平片,随后处死取材,进行大体标本观察、Masson染色及苏木精-伊红染色组织学观察。结果与结论:X射线片与大体标本可见兔桡骨缺损逐渐修复,新生骨走向成熟骨。术后4周,苏木精-伊红染色在材料与骨的结合部及中间的材料上均形成大量新生软骨,有类骨样组织形成及有少量编织骨出现;Masson染色主要表现为绿色,可见到少许红染。术后8周,苏木精-伊红染色出现更多的新生骨组织,并更趋于成熟,出现了成熟的骨细胞及大量编织骨,有血管形成;Masson染色主要表现为红-绿相间。12周时,苏木精-伊红染色新生骨逐渐改建,形成髓腔,有大量板层骨相互融合成片;Masson染色主要表现为红色。说明Masson染色结合苏木精-伊红染色能较好判定新生骨走向成熟骨的过程,但在判定骨质成熟度时Masson染色较苏木精-伊红染色更直观。
BACKGROUND：Hematoxylin-eosin staining and Masson staining are two main methods that are commonly used for evaluating bone tissue repair, but there is a lack of the relevant research on whether these two methods can effectively evaluate the maturity of the bone tissue in the process of bone defect repair. OBJECTIVE：To compare the sensitivity of Masson staining and hematoxylin-eosin staining on the determination of bone tissue maturity. METHODS：New Zealand white rabbits were involved in the study for establishing models of radial bone defects （15 mm in length）. The tissue engineered bone made up of silk fibroin/hydroxyapatite/bone marrow stromal cells was implanted into bone defects in rabbits. The X-ray plain films of six rabbits were randomly selected at 4, 8 and 12 weeks respectively after operation for the general observation, Masson staining and hematoxylin-eosin staining, then the animals were sacrificed. RESULTS AND CONCLUSION：X-ray films and general specimens showed that bone defects were repaired and the new bone was changed into mature bone. At week 4, hematoxylin-eosin staining showed that a large number of new cartilages, some bone-like tissue and a small number of woven bones could be seen around the silk fibroin/hydroxyapatite grafts; Masson staining showed green with a little red dye. At week 8, a lot of new bone, mature osteoblasts and a large number of woven bones were observed by hematoxylin-eosin staining, and the blood vessel formation could be seen. The typical red-green and white views were observed by Masson staining. At week 12, hematoxylin-eosin staining showed that a large number of lamellar bones were integrated into a block, and the new medullary cavity could be seen; Masson staining showed mainly in red. Combination of hematoxylin-eosin staining and Masson staining is a better method to determine the process of new bone to mature bone. However, in the judgment of bone maturity, Masson staining is more intuitive than hematoxylin-eosin staining.
出处
《中国组织工程研究》
CAS
CSCD
2012年第37期6841-6846,共6页
Chinese Journal of Tissue Engineering Research
基金
江苏省骨外科临床医学中心资助项目
国家自然科学基金资助项目(81171689)~~
