摘要
目的通过测定骨形态发生蛋白-7(bone morphogenetic protein-7,BMP-7)、转化生长因子-β1(transforminggrowth factor-β1,TGF-β1)、碱性成纤维细胞因子(basic fibroblast growth factor,bFGF)水平变化,探讨丹参促进骨折愈合在分子水平的作用机制。方法选取72只新西兰大白兔,采用单纯随机抽样方法分为实验组和对照组,每组36只,制作左前臂桡骨骨折模型。实验组肌注丹参注射液(每天1次,每次2 mL),对照组注射生理盐水(每天1次,每次2 mL),至术后28 d。2组分别于术后第1、2、3、4、6、8周选取6只大白兔,X线片观察骨折愈合情况,ELISA法检测其血清BMP-7、TGF-β1和bFGF浓度,处死后取以骨折处为中心的4 mm组织标本,HE染色和免疫组织化学方法检测BMP-7、TGF-β1和bFGF在局部组织中的表达。结果①术后第3、4周X线片显示,与对照组比较,实验组骨痂生成量增加,且达到骨性愈合的时间较早。②ELISA法检测结果显示,术后第4周实验组BMP-7血清含量明显高于对照组(P<0.01);第2周实验组bFGF血清含量明显高于对照组(P<0.05);第3周实验组TGF-β1血清含量明显高于对照组(P<0.05)。③HE染色结果显示,术后第3、4周实验组骨痂形成增多,成骨细胞和破骨细胞数量均较对照组多。④术后第3周实验组BMP-7免疫组化平均光密度值明显大于对照组(P<0.05);术后第2周实验组bFGF免疫组化平均光密度值明显大于对照组(P<0.01);术后第3周实验组TGF-β1免疫组化平均光密度值明显大于对照组(P<0.05)。结论丹参注射液能促进骨折愈合过程中BMP-7、TGF-β1及bFGF生长因子的表达。
Objective To observe the changes of bone morphogenetic protein-7 （ BMP-7 ）, trans- forming growth factor-β1 （ TGF-β1 ） and basic fibroblast growth factor （bFGF） during the healing of fracture in rabbits treated with Danshen injection （ an injection containing Salvia miltiorrhiza extract）. Methods Seventy- two New Zealand White rabbits were made into fracture models and were randomly divided into an experimental group （Danshen injection, 2 mL/d for 28 d） and a control group （normal saline, 2 mL/d for 28 d）. The healing of fracture was evaluated by X-ray and the serum levels of BMP-7, TGF-β1 and bFGF were determined by ELISA at 1, 2, 3, 4, 6 and 8 weeks after the operation. Tissues of fracture region were stained by HE staining, and immunohistochemical method was used to detect the expression of BMP-7, TGF-β1 and bFGF. Results X-ray results showed that as compared with the control group, bone union appeared earlier and more bone callus formed in the experimental group at 3 and 4 weeks. HE staining results showed that more bone callus formed and more osteoblasts and osteoelasts appeared at 3 and 4 weeks in the experimental group than in the control group. ELISA results showed the serum levels of BMP-7 （4 weeks）, TGF-β1 （2 weeks） and bFGF （3 weeks） were significantly higher in the experimental group than in the control group, while immunohisto- chemical staining displayed that the expression levels of BMP-7 （3 weeks） , TGF-β1 （2 weeks） and bFGF （3 weeks） were significantly higher in the experimental group than in the control group. Conclusion Dansh-en injection can upregulate lhe expression of BMP-7, TGF-β1 and bFGF during the healing of fracture.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2013年第3期223-227,共5页
Journal of Third Military Medical University
基金
重庆市卫生局医学科学技术研究项目(2008-2-38)~~
