摘要
目的探讨槲皮素对肺动脉高压的预防及治疗作用。方法将成年雄性SD大鼠40只随机分为模型组、预防组、治疗组及对照组各10只。前三组采用野百合碱(MCT)诱导制作肺动脉高压模型,对照组注射等量生理盐水。制模后模型组及对照组予生理盐水2 mL/d灌胃,预防组制模同时、治疗组制模20 d予槲皮素100 mg/(kg.d)灌胃,均为20 d。干预后测定各组平均肺动脉压力(mPAP)、肺小动脉管壁厚度(WT)占动脉外径(ED)的百分比(WT%)及管壁面积(WA)占血管总面积的百分比(WA%);采用免疫组化和Western blot法检测肺小动脉平滑肌细胞Bcl-2表达。结果与对照组、预防组和治疗组比较,模型组mPAP、WT%、WA%和Bcl-2%显著升高(P<0.05),Bcl-2的表达显著增多(P<0.05)。结论槲皮素对MCT诱导的大鼠肺动脉高压有预防和治疗作用,其机理可能是通过下调肺血管中小动脉平滑肌细胞Bcl-2表达,促进细胞凋亡,改善肺血管重构。
Objective To investigate the prevention and treatment effects of quercetin on pulmonary arterial hyperten- sion （PAH）. Methods Forty male Sprague-Dawley （SD） rats were randomly divided into four groups with ten rats in each group： model group, quercetin-prevention group （P group）, quercetin-treatment group （T group） and control group. The former three groups were made PAH by the induction of monocrotaline（ MCT）, the control group was given the same amount of saline. The model group and control group received saline 2 mL/d by gavage after the molding, the P group and T group received quercetin 100 rag/（ kg ~ d ）by garage at the same time of molding or 20 ds after, and both for 20ds. The pulmo- nary arterial pressure （mPAP） of each groups was measured, Percentage of wall thickness（ WT% ） and Percentage of wall area（ WA% ） of pulmonary arterioles were evaluated; the expression of Bcl-2 in pulmonary arterial smooth muscle was ob- served by immunohistochemistry staining and western blot. Results Compared with the C group,P group and T group, The mPAP, WT% ,WA% and Bcl-2% in MCT group increased significantly（ P 〈 0.05 ）. The expressions of Bcl-2 in MCT group also increased significantly （ P 〈 0.05 ） than other groups. Conclusions Quereetin has the role of prevention and treatment on the MCT-induced PAH in rats , its mechanism maybe due to reverse pulmonary vascular remodeling through down-regulation of Bcl-2 and promote apoptosis in smooth muscle cells.
出处
《山东医药》
CAS
2012年第39期44-47,共4页
Shandong Medical Journal
基金
广东省科技计划项目(kz1011)
