摘要
目的:研究槲皮素-7-硫酸酯钠盐(SQMS)对血栓形成、全血血小板聚集和血小板释放一氧化氮(NO)的影响。方法:用血栓生成仪观察大鼠颈动脉血栓形成情况;小鼠尾静脉注射胶原蛋白-肾上腺素混合诱导剂造成瘫痪模型,观察15min后小鼠瘫痪或死亡数;全血与药物孵育5min,然后用凝血酶或ADP作用1min,用全血血小板聚集仪观察全血血小板聚集情况;血小板悬液与药物孵育5min,然后用凝血酶作用1min,用NIKON TE2000-E荧光倒置显微镜观测血小板聚集情况;用硝酸还原酶法观察血小板释放NO情况。结果:SQMS 10mg/kg和20mg/kg抑制大鼠颈总动脉血栓形成,其抑制率分别为72%和92%;SQMS 20mg/kg和40mg/kg对混合诱导剂造成小鼠瘫痪或死亡有抑制作用,其抑制率分别为70%和80%;SQMS 160、320和640μmol/L抑制凝血酶和ADP诱导的全血血小板聚集,其抑制率分别为24%、76%5、7%和26%5、6%、38%;荧光倒置显微镜观测到血小板聚集的抑制作用随浓度的加大而增强;SQMS 80、160、320和640μmol/L对凝血酶诱导血小板释放NO有加强作用,NO的增加率分别是55%、118%、219%和394%。结论:SQMS对血栓形成有抑制作用,其作用机理与其抑制全血血小板聚集和增加血小板释放NO有关。
Objective：To study the effect of sodium quercetin-7-sulfate（SQMS） on thrombosis,platelet aggregation in whole blood and release of nitric oxide（NO） in platelets.Methods：The thrombosis in the carotid artery of rat was observed by a thrombosis inducer.Thrombosis of mice was induced by tail intravenous injection of a mixture of collagen and adrenaline and the number of paralysis or dead after 15 min was also calculated.Whole blood was incubated with SQMS for 5 min,and then was treated with thrombin or ADP for 1 min.Platelet aggregation was analysed by whole blood aggergometers.Suspensions of platelets were incubated with SQMS for 5 min,and then were treated with thrombin for 1 min.Platelet aggregation was observed by inverted microscope（NIKON TE2000-E）.Release of NO in platelets was measured by nitrate reductase-dependent assay.Results：SQMS 10mg/kg and 20mg/kg inhibited thrombosis in the carotid artery of rat with inhibition rate 72% and 92%,respectively.SQMS 20mg/kg and 40mg/kg could inhibit the number of paralysis or dead induced by the mixture of collagen and adrenaline with inhibition rate 70% and 80%,respectively.SQMS 160、320 and 640μmol/L inhibited platelet aggregation in whole blood induced by thrombin and ADP with inhibition rate 24%、76% 、 57% and 26%、56%、38%,respectively.Enhanced inhibition on platelet aggregation with incremental concentration of SQMS was observed by inverted microscope.SQMS 80、160、320 and 640μmol/L had enhanced release of NO in platelets induced by thrombin with NO increase rate 55%、118%、219% and 394%,respectively.Conclusion：SQMS showed inhibition on thrombosis and its molecular mechanism was due to its inhibition on platelet aggregation in whole blood and release of NO in platelets.
出处
《中药药理与临床》
CAS
CSCD
北大核心
2011年第3期30-33,共4页
Pharmacology and Clinics of Chinese Materia Medica
基金
广东省重点学科基金资助(NO:9306)
