摘要
目的优化埃博霉素B发酵培养基。方法以纤维堆囊菌SoF5-9为供试菌株,通过单因素和正交试验研究了埃博霉素B合成的最佳培养基。结果埃博霉素B最适发酵培养基为糊精0.3%,蔗糖0.07%,葡萄糖0.02%,豆饼粉0.17%,MgSO4.7H2O 0.1%,无水氯化钙0.1%,EDTA-Fe3+2mL/L,微量元素(TE)0.5mL/L。在该最优条件下,埃博霉素B产量可达到27.5mg/L。结论合理优化发酵培养基组分可以有效提高埃博霉素B的产量。
Objective Optimizing epothilone B fermentation medium. Method The medimn of Sorangium cellulosum SOF5-9 for the production of e- pothilone B was optimized by single factor experiment and orthogonal design method. Result The optimum fermentation medium of epothilone B was as followed： dextrin 0.3%, sucrose 0.07%, glucose 0.02%, soybean cake powder 0.17%, MgSO4· 7H20 0.1%, CaCI2 0.1%, EDTA-Fe3. 2mL/L, TE 0.5mL/L. Under the optimal condition, the production of epothilone B reached 27.5mg/L. Conclusion Optimizing fermentation medium can effectively improve the production of epothilone B.
出处
《中国酿造》
CAS
2012年第10期30-32,共3页
China Brewing
基金
国家自然科学基金(No.20906058)
陕西省自然科学基金(No.2010JQ4020)
陕西省教育厅自然科学研究专项(No.12JK1023)
陕西省科技厅社会发展攻关项目(No.2010K01-200)
陕西科技大学学术骨干培育项目(No.XSG2010009)
关键词
纤维堆囊菌
埃博霉素B
培养基优化
正交试验
Sorangium Cellulosum
epothilone B
medium optimization
orthogonal experiment
