摘要
对实验室现有3种真菌产纤维素酶能力的分析及培养条件优化。比较了3种菌在刚果红培养基上的透明圈大小、并分析产纤维素酶酶活;通过单因素与响应面分析的方法优化毛酶产纤维素酶的培养条件。通过试验得出3种真菌均能产纤维素酶,毛霉能产较多的纤维素酶。毛霉产纤维素酶的最佳条件为:pH 5.0,转速220 r/min,发酵时间47 h,发酵温度35℃,纤维素酶活为6.99U/mL。毛霉、青霉、曲霉均产纤维素酶,毛霉能降解玉米芯纤维素。
We analyzed the cellulose degradation ability of three kinds of fungi （ Penicillium, Mucor and AspergilhL ） preserved in the laboratory and optimalized the culture conditions. The transparent ring size of three kinds of fungi training in the congo red medium and in the corncob congo red medium was observed. The cellulase activity was measured of the three fungi cultured in the CMC-Na medium. Single factor and response surface methodology were used to optimize the culture condition. The three kinds of fungi all could produce cellulase. The cellulase activity of Mucor was higher than the other two cultured in the the CMC-Na medium. The optimized conditions were below ： pH was 5.0, the shaking speed was 220 r/min, fermentation time was 47 h, fermentation temperature was 35 ℃. The highest CMCase activity was 6.99 U/mL training on the optimized condition. Penicillium, Mucor and Aspergillus produced cellulase and Mucor could degrade corncob.
出处
《生物技术通报》
CAS
CSCD
北大核心
2014年第1期182-187,共6页
Biotechnology Bulletin
关键词
霉菌
纤维素酶
培养条件
优化
Fungi Cellulase Culture condition Optimize
