摘要
采用响应面法对地衣芽孢杆菌产微生物絮凝剂发酵培养基进行优化,以期提高微生物絮凝剂的活性和产量。首先利用Plackett-Burman实验设计筛选出影响微生物絮凝剂絮凝活性的三个显著因素:大豆油,氯化钙,硫酸锰;并以此为基础,利用最陡爬坡实验接近响应值的附近区域;最后通过中心复合实验和响应面分析确定了影响微生物絮凝剂絮凝活性主要因素的最佳配方浓度,分别为大豆油0.49%、豆粕5.0 g/L、磷酸二氢钾0.2 g/L、氯化钙溶液(7.5 g/L)26μL/L、硫酸锰溶液(2.0 g/L)260μL/L、硫酸亚铁溶液(2.0 g/L)100μL/L。此时微生物絮凝剂絮凝剩余浊度的预测值为28.1 NTU。通过模型的验证实验发现,微生物絮凝剂的实际絮凝活性为28.6 NTU,与模型预测值非常接近,并且絮凝剂产量达到1.05 g/L,比优化前提高了26.51%。
The response surface methodology was applied to optimize the fermentation medium in order to improve the activity of microbial flocculants by Bacilluslicheniformis. First,soybean oil,Ca Cl2 and Mn SO4 were found to be significant factors influencing the production of microbial flocculant,which was obtained by the Plackett- Burman screening design. The steepest ascent experiment aiming at approaching the optimum region of the response was carried out subsequently.Finally,the effect of the three significant factors on microbial flocculant production were further investigated by a central composite design and the optimal condition was determined as follows：0. 49% soybean oil,5. 0 g / L soybean meal hydrolysate,0. 2 g / L K2PO4,26 μL/L calcium chloride solution（ 7. 5 g / L）,260 μL / L manganese sulfate solution（ 2. 0 g / L） and 100 μL / L ferrous sulfate solution（ 2. 0 g / L）. With the optimal condition,predicted highest the activity of microbial flocculants of 28. 1 NTU would be reached. An actual value of 28. 6NTU was obtained in the verification experiment,which was in agreement with the prediction. The production of microbial flocculant reached up to 1. 05 g / L and was increased by 26. 51% compared with that using the original culture medium.
出处
《盐业与化工》
CAS
2016年第10期24-29,共6页
Jounral of Salt and Chemical Industry
基金
海洋公益性行业科研专项项目(201405017-04)
中央级公益性科研院所基本科研业务费专项资金项目(K-JBYWF-2016-G10)
关键词
地衣芽孢杆菌
微生物絮凝剂
响应面
培养基优化
bacilluslicheniformis
microbial flocculants
response surface methodology
medium optimization
