摘要
为了确定一株筛选自低温污水的产蓝色素细菌B9-8分类地位和其色素的特性,通过对菌株的形态观察和16S rRNA基因序列比对分析,确定菌株B9-8归属为Janthinbacterium属菌,可能为新种。色素特性研究表明:该蓝色素在可见光区579nm波长处有最大吸收峰,具有较好的水溶性和有机溶性,较好的热稳定性和酸碱稳定性,但光稳定性较差,在自然光条件下放置3d色素损失61.3%。低浓度金属离子对色素的影响不大,而高浓度的金属离子,尤其是Al3+对色素的影响较大,损失率达73.1%,推测此色素极可能为紫色杆菌素。
Purposes： To identify the classification position of a blue pigment producing strain named as B9-8 isolated from low temperature sewage and to characterize pigment produced by it. Methods： Based on morphological observation and 16 S rRNA sequence alignment, the strain was identified as a possible new member of the genus Janthinbacterium. Results： The blue pigment from this strain showed a maximum absorption peak at 579 nm and had good solubility in water and organic solvents. In addition, it was stable to heat and pH but unstable to light. After 3 d of exposure to natural sunlight a loss of 61.3% was found. Low concentrations of metal ions had little effects on its stability, whereas some metal ions at high concentrations, especially A1^3＋, had a great impact on its stability, resulting in a loss as high as 73.1%. Conclusion： Therefore, we speculated that the blue pigment might be violacein.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2012年第19期236-239,共4页
Food Science
基金
新疆维吾尔自治区自然科学基金项目(2010211B28)
农业部公益性行业(农业)科研专项(200803034)
关键词
蓝色素
菌种鉴定
特性
稳定性
blue pigment
strain identification
properties
stability
