摘要
目的:从土壤中筛选绿色素产生菌株并对该菌株进行鉴定。方法:采用平板法分离产绿色素菌株,利用形态、生理生化及分子生物学的方法对菌株进行菌种鉴定。结果:从四川理工学院校园土壤中分离得到一株产绿色素的放线菌t9,在高氏一号培养基上,气生菌丝为灰白色,基内菌丝为绿色。该菌株基因序列与鼠灰链霉菌Streptomyces_murinus_NBRC_12799(T)相似度为99%,以Neighbor-Joining(N-J)法构建的系统发育树。结论:菌株t9的16S r DNA序列分析结果结合形态学特征及生理生化鉴定,确定该菌株为鼠灰链霉菌属(Streptomyces_murinu),并将其命名为Streptomyces_murinus t9。
Objective： In order to isolation and identification of strain of producing green pigment. Methods：The microbial strains of producing pigment were isolated from soil by method with plate coating,biochemical and morphological characteristics and 16S rDNA gene sequence analysation. Results：An actinomycete,named t9, was isolated from a soil sample in the College of Bioengineering,Sichuan University of Science and Engineering. It produced grayish-white aerial mycelium and green substrate mycelium on Gause＇s synthetic agar. The results showed that the 16S rDNA gene sequence of the train t9 shared the identity 99% with that of Streptomyces_murinus__NBRC_ 12799（T）,the phylogenetic tree was derived with NJ. Conclusion =The strain t9 was identified as Streptomyces_murinus from its morphological andcultural characteristics and the 16S rDNA gene sequence.
出处
《食品工业科技》
CAS
CSCD
北大核心
2015年第20期188-190,196,共4页
Science and Technology of Food Industry
基金
四川省教育厅成果转化项目(11ZZ016)
关键词
绿色素
链霉菌
筛选
green pigments
streptomyces
screening
